应用252条10-mer随机引物对遗传背景相似的可育株和不育株亚麻进行了RAPD分子标记,在不育株与可育株之间寻找DNA的多态性差异带,结果发现,在252条随机引物中有2条引物(即S62和S135)可分别得到1个与显性核不育的雄性基因有关的RAPD分子标记为S62-500和S135-350。
A total 252 arbitrary 10-mer primers were used to screen the dominant male genomic sterile and male fertile gene in flax.Two primers were found,S62 and S135,respectively,could produce RAPD markers correlated with dominant genomic male sterile gene,which are S62-500 and S135-350.