中文摘要：

目的探讨miR-324-3p对鼻咽癌细胞体外迁移侵袭能力的影响。方法 MiR-324-3p转移上调其在鼻咽癌5-8F和6-10B细胞中的表达,采用划痕愈合实验及Transwell侵袭实验检测上调后的鼻咽癌5-8F和6-10B细胞迁移及侵袭能力;western blot检测上皮-间质转化（EMT）上皮细胞标志物E-cadherin和间质细胞标志物Vimentin的表达。结果 MiR-324-3p成功转移上调了其在鼻咽癌5-8F和6-10B细胞中的表达。过表达miR-324-3p的鼻咽癌5-8F和6-10B细胞的体外迁移及侵袭能力显著下降,可同时伴随着E-cadherin的表达上调,Vimentin的表达下降。结论 MiR-324-3p可能通过诱导EMT改变调控鼻咽癌细胞的侵袭转移能力。

英文摘要：

Objective To investigate the effect of miR-324-3p on the migration and invasion in nasopharyngeal carcinoma（ NPC） in vitro. Methods MiR-324-3p mimic was used to up-regulate the expression of miR-324-3p in NPC 5-8F and 6-10B cell lines,and then alterations of migration and invasion were examined by wound healing assays and Transwell invasion assays. Molecular markers correlated with epithelial-mesenchymal transition（ EMT） were finally tested.Results The expression of miR-324-3p was successfully up-regulated in both NPC 5-8F and 6-10B cells. Moreover,miR-324-3p overexpression led to enhanced capabilities of migration and invasion in NPC 5-8F and 6-10B cells,which was accompanied by elevated expression of E-cadherin and decreased expression of Vimentin. Conclusion MiR-324-3p can regulate the migration and invasion of NPC via EMT.