中文摘要：

目的：探讨不同剂量的表没食子儿茶素没食子酸酯（EGCG）对哺乳期铅暴露仔鼠学习记忆能力的修复作用以及海马组织抗氧化损伤作用的影响。方法：选取10只SPF级C57BL/6孕鼠,分为对照组（2只）和染铅组（8只）。染铅组孕鼠自分娩第1天至第20天经饮水染铅（饮用5 g/L的醋酸铅溶液）,对照组饮用蒸馏水,母鼠通过乳汁对F1代仔鼠染毒。染铅结束后,取40只染铅仔鼠分为低、中、高剂量EGCG干预组、染铅模型组,每组10只,10只未经染铅的仔鼠作空白对照,分别用不同浓度的EGCG溶液和生理盐水等体积灌胃21 d。用Morris水迷宫和抗氧化酶试剂盒分别检测仔鼠学习记忆能力和海马组织中SOD、GSH-Px活性和MDA含量。结果：各组小鼠平均逃避潜伏期差异有统计学意义（P均＜0.001）; 与染铅模型组相比,中、高剂量EGCG干预组SOD、GSH-Px活性明显升高,MDA含量明显降低（P＜0.05）。结论：EGCG通过提高抗氧化酶（SOD和GSH-Px）活性拮抗铅暴露引起的氧化损伤,改善仔鼠学习记忆能力。

英文摘要：

Aim： To explore the effects of epigallocatechin gallate（EGCG）on spatial learning memory performance and antioxidative damage ability in lead exposed offspring mice during lactation.Methods： A total of ten healthy pregnant C5BL/6 mice were randomly allocated into control group and lead exposed group, which were treated with drinking double evaporated water and Pb containing water, respectively from the beginning of offspring birth and lasted until weaning. There were 2 pregnant mice in control group and 8 pregnant mice in lead exposed group. The offspring mice were exposed to lead by maternal milk. Forty lead exposed offspring mice and ten control offspring mice were employed as the research objects. According to the body weight,the mice were stochastically allocated into five groups： lead exposed group（normal saline）,low,middle,high dose EGCG interfering groups and control group（ten control offspring mice）and each group was administered for by lavage 21 d.The spatial learning and memory ability was evaluated by Morris water maze,SOD and GSH-Px activity levels and MDA level in hippocampus tissue were measured by corresponding kits.Results： The escape latency in each group measured at different days had significant differences and there were also differences between different groups（P〈0.001）; the activity levels of SOD and GSH-Px were higher in middle,high interfering groups than those in lead exposed group, while the content of MDA was lower（P〈0.05）.Conclusion： EGCG may protect offspring mice from lead-induced peroxidation and enhance the spatial learning and memory ability through improving antioxidant enzymes activity.