中文摘要：

目的：为了从遗传表达角度解析两种新疆紫草细胞系中紫草素类化合物红色系高产和白色系低产的原因。方法：采用Real—timePCR的方法获得不同生长时期新疆紫草红色高产系和白色低产系紫草素生物合成关键酶基因的表达规律及相对表达量差异。结果：新疆紫草红色高产系和白色低产系在不同生长时期其关键酶基因（HMGR、PAL、4-CL、C4H、GDPS、PGT）的表达水平除个别时间段的个别基因外，红色高产系生长周期内紫草素生物合成关键酶基因的表达量均远大于白色低产系，其中以生长旺盛的第5～17天尤为明显。在不同生长时期其关键酶基因的表达随生长周期时间变化而波动，红色高产系中关键酶基因的表达随时间变化的曲线为倒u型，而白色低产系中此曲线呈U型趋势。结论：新疆紫草白色低产系同样具备有合成紫草素类化合物的潜力，并且白色系紫草素类生物合成途径的障碍发生位置应当在PGT酶促反应的下游。

英文摘要：

Objective： To Analysis the cause about high-yielding of red shikonin-proficient line and low-yielding of white shikonin-deficient line in terms of genetic expression. Method： The expression differences of HMGR, PAL, 4-CL, C4H, GDPS and PGT, which involved in shikonin biosynthesis, in different cultures and different growth stages cultured in fresh medium were detected by Real-time PCR method. Result： It is found that the expression level of key enzyme genes （HMGR, PAL, 4-CL, C4H, GDPS, PGT） in red shikonin-proficient line was much higher than the white shikonin-deficeint line mostly, except some individual genes in the individual time periods and it was much higher especially at 5 to 17 days with vigorous growth. And the relative expression level of genes in shikonin-proficient and deficient lines of A. euchroma fluctuate over time during the growth Cycle. Separately, relative expression level of genes in red shikonin-proficient line changed with time as an inverted U-curve, but this curve was U-shaped in white shikonin-deficiect line. Conclusion： It could be inferred that white line of Arnebia euchroma had potential for shikonin synthetizing and its barriers might located at somewhere downstream after PGT enzymatic reaction of shikonin biosynthetic pathway. This study made the foundation of gene discovering in shikonin biosynthesis pathway, as well as molecular biology regulation of shikonin production.