中文摘要：

目的：探讨高频正弦波振动（HFV）对骨骼肌在废用状态时细胞凋亡的对抗作用。方法：建立废用动物模型,改进振动仪的关键技术,采用短时间歇式振动的方法,结合电镜观察、免疫组化染色法和TUNEL法检测,观察HFV对制动大鼠比目鱼肌（SOL）湿重体重比、肌纤维横截面积、肌纤维超微结构的变化、Bcl-2和Bax的表达情况。结果：（1）制动后大鼠SOL湿重体重比为30.22±2.68,下降了36.43%。肌纤维的横截面积为3789.56±95.14μm2,下降了37.77%。HFV组相对制动组的SOL湿重体重比及横截面积均有所增加（P〈0.05）;（2）电镜可见制动后梭外肌病变明显,核内染色质团块状凝集。梭内肌部分肌膜溶解消失,细胞核变形。振动组的梭外肌Z线结构比较清晰,梭内肌纤维结构未见明显改变;（3）制动后大鼠SOL中Bcl-2阳性表达率为20%,明显低于对照组的表达情况（P〈0.05）。制动后Bax阳性表达率为80%,明显高于对照组的表达情况（P〈0.05）。制动后AI为20%,明显高于对照组（P〈0.05）。HFV组相对制动组的Bcl-2阳性表达率均有所增加,Bax阳性表达率以及AI有所降低（P〈0.05）。结论：改进后的抗肌萎缩振动仪操作性较强,对制动大鼠废用性肌萎缩的凋亡进程有抑制作用。

英文摘要：

Objective： To investigate the antagonism of high -frequency sine -wave vibration （HFV） on the apoptosis of skeletal muscle in the disused state. Methods： The animal models were established and the key techniques of the vibration instrument were improved. The short - term intermittent vibration was used to observe the effects of HFV on soleus muscle （SOL） wet weight in the brains of SD rats. The ultrasonography, immunohistochemical staining TUNEL assay, the effect of HFV on the brake in rat soleus muscle （SOL） wet weight to body weight ratio, muscle fiber cross - sectional area, ultrastructural changes, Bcl - 2 and Bax expressiott. Results 1 ） The body weight ratio of SOL was 30.22 ＋ 2.68, decreased by 36.43%. The cross - sectional area of muscle fiber was 3789.56 ：t： 95.14-m-, which decreased by 37.77%. （P 〈 0.05 ）. （2） Electron microscopy showed that the extraocular muscle lesion was obvious after the brachytherapy, and chromatin agglutination in the nucleus was observed in the HFV group compared with the brachytherapy group. Skeletal muscle part of the dissolution of the sarcolemma, nuclear deformation. （P 〈 0.05）, the Z - line structure of the extra - oculi muscle in the vibration group was clear, and no obvious change was observed in the intrafusal muscle fiber structure. 3 ） The expression of Bcl - 2 in the SOL was significantly lower than that in the control group 〈 0.05 ）. The positive rate of Bax expression was 80% after brakes, which was significantly higher than that of the control group （P 〈 0.05）. AI after braking was 20%, significantly higher than the control group （P 〈 0.05 ）. The expression of Bcl -2 and the expression of Bax and AI in the HFV group were significantly lower than those in the control group （P 〈 0.05 ）. Conclusion The modified dystrophy vibrator has strong operability, which can restrain the process of apoptosis of the brake muscular atrophy.