中文摘要：

目的 探究spycatcher可作为一种新的高效促溶标签蛋白。方法 通过分子生物学方法将spycatcher基因与包涵体PD1及MPD1基因融合构建到表达载体PET21a中,然后通过ELISA和fortebio验证其融合蛋白活性。结果 spycatcher与包涵体靶蛋白PD1及MPD1融合后为可溶性表达,融合蛋白纯化后能够与其配体PDL1有效结合。结论 Spycatcher可作为一种新的高效促溶标签蛋白,这将为不可溶表达蛋白的表达纯化带来诸多便利。

英文摘要：

Objective To explore if spycatcher can be used as a new efficient solubilization of tagged protein. Methods Fusing the genes of spycatcher with Pdl and MPDl（inclusion-body proteins） into the expression vector of PET21a using molecular biology method, the activity of the fusion proteins was verified by ELISA and fortebio. Results The fused protein of spycatcher and Pd 1 and MPD 1 was soluble and effectively affinity with its ligand PDL1. Conclusion Spycather can be used as a new high efficient soluble lable protein, was convenience for the expression and purification of insoluble protein.