中文摘要：

该文建立了大孔树脂-高速逆流色谱分离薇甘菊中黄酮类物质的方法.分离条件为：采用大孔树脂AB-8,洗 脱液为50% （v/v）乙醇水溶液,高速逆流色谱溶剂体系为正丁醇-乙酸-水（4：1：5, v/v）.从薇甘菊中分离到4种黄 酮类物质：槲皮素-3-O-芸香糖苷（纯度90. 2%）、山奈酚-3-O-芸香糖苷（纯度98.55%）、木犀草苷（纯度98.33%）和 紫云英苷（纯度99.23%）.建立的大孔树脂-高速逆流色谱方法简单、高效,可扩展应用于从其他植物中分离黄酮类物质.

英文摘要：

The present study established a method for the separation and purification of four flavonoids quercetin-3-O- rutinoside, kaempferol-3-O- rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography （HSCCC）. Macroporous resin was AB-8 and the eluent was 50% （v/v） ethanol. A two-phase solvent system composed of nbutanol/acetic acid/water （4：1：5, v/v） was used in HSCCC. Quercetin-3-O- rutinoside, kaempferol-3-O- rutinoside, luteoloside and astragalin with the purity of 90. 12%, 98. 55%, 98. 33% and 99. 23%, respectively, were successfully separa-ted from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.