中文摘要：

目的了解转化生长因子（TGF）β及结缔组织生长因子（CTGF）对大鼠肝脏前体细胞（WB-F344）的作用。方法不同浓度的重组人TGFβ和CTGF直接刺激WB-F344细胞，观察两种细胞因子对细胞活性的影响，检测间质细胞活化标志物α-平滑肌激动蛋白（α-SMA）和金属基质蛋白酶抑制物（TIMP）-1、I型胶原（collagenI）、Ⅲ型胶原（collagen Ⅲ）等细胞外基质标志物mRNA的表达。检测α-SMA的蛋白表达。观察α-SMA的免疫荧光表达。结果TGFβ及CTGF对WB-F344细胞的增殖均有抑制作用，其中TG即的抑制作用更明显。10ng／mLTG即可显著提高前体细胞α-SMA、TIMP-1、collagenI、collagenIII的mRNA的表达，分别为对照组的10．1、3．0、5．0和5．1倍（P〈0．05），而CTGF对上述指标影响不大。在蛋白水平上，TGFβ和CTGF均可增加前体细胞表面α-SMA蛋白的表达。免疫表型方面，1ng／mL和10ng／mL的TGFβ及CTGF均可增强α-SMA的荧光强度。结论TGFβ及CTGF均可抑制大鼠肝脏前体细胞增殖，并增加α-SMA蛋白及荧光的表达，而在α-SMA、TIMP-1、collagenI、collagenIII的mRNA表达上二者作用不同。

英文摘要：

Objective Transforming growth factor β（TGFβ）and its downstream cytokine connective tissue growth factor（CTGF）have close relationship with liver fibrosis. The expression of these two cytokines has positive correlation with the activation of stellate cells and fibrosis. However, whether TGFβ and CTGF have the similar effects on liver progenitor cells is not clear. This research aims to compare the effects of TGFβ and CTGF in rat hepatic progenitor cells（WB-F344 cells）. Methods The influence of TGFβ and CTGF on the viability and morphology of WB-F344 cells was evaluated. The mRNA expressions of α-smooth musle actin（α-SMA）, tissue inhibitor of metalloproteinase（TIMP-1）, collagen I, collagen III of WB-F344 cells induced by TGFβ and CTGF were analyzed. The protein expressions of α-SMA was observed. Immunofluorescence was used to detect the expressions of α-SMA. Results Both TGFβ and CTGF could reduce the proliferation of WB-F344 cells. TGFI3 has greater suppression function than CTGF does. The dose of 10 ng/mL of TGFβ could improve the mRNA expressions of α-SMA, TIMP-1, collagen I and collagen III significantly, while the same dose of CTGF has little influence on mRNA expressions. Both doses of 1 ng/mL and 10 ng/mL of TGFβ and CTGF could improve the protein expression and fluorescence brightness of α-SMA. Conclusion Both TGFβ and CTGF could suppress the cell proliferation ,improve α-SMA protein expression and fluoreseence brightness in rat hepatic progenitor cells. But these two cytokines have different effects on the mRNA expressions of α-SMA, TIMP-1, collagen I and collagen III.