中文摘要：

目的：观察大鼠肝脏前体细胞系（WB-F344）对肝星状细胞系（HSC-T6）活化及细胞外基质的影响。方法将慢病毒-GFP 空白载体转染的 HSC-T6与 WB-F344按1∶1与1∶2比例直接共培养3 d，使用流式细胞仪对其进行分选，慢病毒-GFP 空白载体转染的 HSC-T6单独培养作为对照，观察 HSC-T6活化指标及细胞外基质相关指标表达的变化。结果经流式细胞仪检验慢病毒-GFP 空白载体转染 HSC-T6的效率可达近90％；与 WB-F344直接共培养3 d 后，HSC-T6的细胞形态与对照组相比无差别；同时利用 GFP 对各组 HSC-T6细胞分选后，经流式细胞仪检验纯度可达94％；对分选后的细胞进行分析发现，直接共培养组 HSC-T6细胞活化标记物α-平滑肌肌动蛋白（α-SMA）在基因水平（1∶1与1∶2组分别是对照组的0．66与0．61倍，P＜0．05）和蛋白水平（1∶1与1∶2组分别是对照组的0．61倍与0．25倍，P＜0．05）的表达均下降；HSC-T6细胞的细胞外基质标记物 I 型胶原（Col-I）、基质金属蛋白酶-13（MMP-13）、金属蛋白酶组织抑制剂-1（TIMP-1）的表达与对照组比较差异无统计学意义。结论WB-F344细胞可以抑制 HSC-T6细胞的活化，但在一定时间内对其细胞外基质的表达没有影响。

英文摘要：

Objective To explore the effects of HPCs （WB-F344）on activation and extracellular matrix （ECM）of HSCs （HSC-T6）.Methods Lentivirus-GFP transfected HSC-T6 was directly cocultured with WB-F344 at 1 ∶1 and 1 ∶2 ratios for 3 days.Mixed cells were sorted by flow cytometry based on the presence or absence of GFP.Transfected HSC-T6 cultured alone was used as control.Expression of activation marker α-smooth muscle actin （α-SMA）and ECM markers collage I （Col-I）,matrix metal proteinase-13 （MMP-13）,tissue inhibitor of metalloproteinase-1 （TIMP）-1 in HSC-T6 were observed by Western blot and real-time PCR.Results After 3 days of coculture with WB-F344,morphological changes of HSC-T6 remained unchanged in direct coculture groups.Transfection efficiency of Lentivirus-GFP was nearly 90% and the purity of sorted HSC-T6 was 94%.WB-F344 directly cocultured with HSC-T6 had reduced α-SMA expression of HSC-T6 in mRNA and protein level.However,there was no difference in ECM expression between control group and direct cocul-ture groups.Conclusion WB-F344 can inhibit the activation of HSC-T6,but it had no influence on expression of ECM.