中文摘要：

目的：预测人Izumo蛋白的二级结构及B细胞抗原表位。方法：以人Izumo基因序列为基础，按Chou-Fasman和Gamier-Robson方法预测其编码蛋白的二级结构，采用Karplus—Schulz方法预测Izurllo蛋白骨架区的柔韧性；按Kyte-Doolittle方法预测其亲水性、Emini方法预测蛋白质表面可能性及Jameson-Wolf方法预测抗原性指数。结果：Chou-Fasman及Gamier-Robson两种方法预测的结果均表明，Izumo蛋白含较多的α螺旋，蛋白第6—17、30～40、88—99、103～120、153—160、173～188、249～260、283．297、334-338和339—346区段可能是Q螺旋中心，第21～25、198～200、245～248和320—323区段可能是8折叠中心。用Kyte-Doolitfle、Emini和Jameson-Wolf方法分别对Izumo蛋白B细胞抗原表位进行预测结果表明，蛋白质第36～42、62～66、94～99、118～122、129～132、151～154、161～164、173～177、205～208、212～216、256～265、271～276、283～288、314—318和336—350区段附近很可能为B细胞表位优势区域。结论：该研究结果有助于确定Izilmo蛋白的B细胞优势表位及发挥免疫避孕的活性部位。

英文摘要：

Objective： To predict and analyze the secondary structure and B cell epitopes of Izumo protein. Methods： The secondary structure and flexible regions of Izumo protein were predicted by the methods of Chou-Fasman, Gamier-Robson and Karplus-Schulz. Moreover, hydrophilicity plot, surface probability plot and antigenic index of Izumo protein were predicted by the methods of Kyte-Doolittle, Emini and Jameson-Wolf, respectively. Results： Izumo protein contained more a helix regions. There were several centers of α helix in the regions of 6-17, 30-40,88-99,103-120,153-160,173-188,249-260,283-297,334-338 and 339-346 of lzumo protein, and several centers of β sheet in the regions of 21-25,198-200,245-248 and 320-323. Moreover, many distinct B cell epitopes in Izumo protein possibly localized in the regions of 36- 42,62-66,94-99,118-122,129-132,151-154, t61-164,173-177,205-208,2!2-216,256-265,271-276,283-288,314-318 and 336-350. Conclusion： These results are helpful for identification of the dominant B cell epitopes and the functional domains of Izumo protein.