中文摘要：

采用新设计COI、16S、CR3种线粒体基因（位点）的简并引物,对江西地区常见蛇类（2科6属7种）共20个个体样本进行了DNA条形码分析的初步尝试,将实验结果结合GenBank部分蛇类序列数据分析表明,在本实验涉及的蛇类个体及类群的识别上,16S rRNA基因片段合乎通用条码标记的序列的要求;且本实验设计的16S简并引物有适用性强、宽容度大的优势。其次,本研究结果支持＂16S rRNA基因至少应该作为脊椎动物标准条码标记———线粒体COI基因不可缺少的补充＂观点;提示当前对DNA条形码在不同生物类群的应用方面,尚待在更大的标本样品数量及更多不同基因片段序列数据的支撑。

英文摘要：

Referred to snake sequence data from GenBank,and studied 20 samples of snakes in Nanchang,which include 2 families,6 genus,and 7 species,to present a first attempt in applying the usage of DNA barcode in the species of snakes.Our experiment demonstrates,in terms of identifying snakes samples or groups,mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker;we also show certain advantage in the application and universality of combination.Furthermore,our result supports the opinion：＂We strongly advocate the use of 16S rRNA as standard DNA barcoding marker for vertebrates to complement COI,especially if samples a priori could belong to various phylogenetically distant taxa and false negatives would constitute a major problem.＂（Miguel et a1.2005）.we recommend richer species samples and larger various gene sequences to support a wider application of DNA barcode in different biological field.