为探索农抗702防治早稻叶瘟和穗颈瘟的田间药效。分别以农抗702和水稻品种早杂两优287为试验材料,在水稻分蘖期和齐穗期喷施不同浓度的农抗702药液进行防治水稻叶瘟和穗颈瘟的研究。防治叶瘟试验,在水稻分蘖期喷施药液前1 d和后15 d分别进行病情调查;防治穗颈瘟试验,分别在水稻乳熟期进行病害调查和水稻成熟测产。并测定相应时期水稻叶片中POD、CAT、SOD、PAL和PPO活力。分别以喷施春雷霉素(45μg/m L)为阳性对照和喷施去离子水为空白对照。喷施15、30和45μg/m L三种浓度的农抗702对叶瘟防治效果分别为72.14%、80.32%和85.22%;对穗颈瘟的防治效果分别为74.08%、84.87%和86.58%;对水稻的增产率分别为17.28%、21.40%和22.57%。由此可见,农抗702能较有效的提高水稻叶片内防御酶活力,防治稻瘟病并提高水稻产量,是防治稻瘟病较理想的新型杀菌剂。本研究可为农抗702的进一步研发及其对稻瘟病的防治应用提供一定的数据支持。
The effects of agricultural antibiotic 702(ag-antibiotic 702) on controlling rice leaf blast and panicle blast were evaluated in field trials. The ag-antibiotic 702 and the two-line hybrid rice 287 were used as test materials. Rice blast controls were researched by spraying different concentration of ag-antibiotic 702 liquor on rice at the tillering stage and heading stage. The disease indexes of leaf blast control were surveyed at one day prior to and fifteen days after spraying liquor at the tillering stage of rice, respectively. The disease indexes(DI) and the rice yield of panicle blast control were investigated at the milk stage. Meanwhile, enzyme activities, including peroxidase(POD), catalase(CAT), superoxide dismutase(SOD), phenylalanine ammonia-lyase(PAL), and polyphenol oxidase(PPO), were assayed. In this study the kasugamycin(45 μg/m L) and deionized water were designed as positive and negative controls. With spraying concentration of 15, 30 and 45 μg/m L of ag-antibiotic 702 liquor, the control efficiencies of leaf blast were 72.14%, 80.32% and 85.22%, respectively; the control efficiencies of panicle blast were 74.08%, 84.87% and 86.58%, respectively; and rates of increase in grain yield were 17.28%, 21.40% and 22.57%, respectively. So, ag-antibiotic 702, as a new agricultural antibiotic fungicide, could effectively control rice blast by producing defensive enzymes, and increase the grain yield. The results will contribute to the further development of ag-antibiotic 702 and its the application to the rice blast control.