中文摘要：

目的：研究杜仲叶总提取物对去势大鼠骨质疏松（osteoporosis，OP）模型的骨代谢调节作用机制。方法4月龄雌性SD大鼠32只，随机分成正常对照组（Control组，生理盐水灌胃）、去卵巢对照组（OP组，生理盐水灌胃）、雌二醇组（Estrogen组，雌二醇水溶液灌胃）、杜仲叶总提取物组（DZ组，杜仲叶总提物水溶液灌胃）。各组治疗3个月后，通过眼眶采血，测定血清中Ca2＋、P3＋和ALPase活力。并取大鼠的股骨和胫骨，用双能X线骨密度仪测定骨密度。结果血清中Ca2＋、P3＋和ALPase活力，DZ组与OP组比较，均有显著差异（P〈0.05）；股骨和胫骨BMD，DZ组与OP组比较，有显著差异（P〈0.05）。结论杜仲叶总提取物可以改善OP模型动物骨代谢，增加骨质疏松模型动物的骨密度，减少骨破坏，加强骨稳定，有效防治骨质疏松症。

英文摘要：

Objective Study of adjusting effect of total extraction in Eucommia ulmoides oliv leaf on bone metabolism in os-teoporosis （OP） model animal’s OP and its mechanism. Methods Thirty-two SD female rats at the age of 4 months were randomly divided into 4 groups：normal control group （gavage with physiological saline）,ovariectomized group （OP group,gavage with physio-logical saline）,estrogen group （gavage with estrogen）,total extraction in Eucommia ulmoides oliv. Leaf group （DZ group,gavage with total extraction of Eucommia ulmoides oliv. leaf）. At the end of 3-month treatment,blood calcium （Ca2＋）,blood phosphor （P3＋）, and alkaline phosphates （ALP） were tested by orbital blood collection. Thon femur and tibia of 4-group animals were taken out. Bone mineral density （BMD） of them was determined using dual-energy X-ray absorptiometry. Results Compared with OP group, blood calcium （Ca2＋）,blood phosphor （P3＋）,and alkaline phosphatase （ALP） in DZ group were decreased significantly （P〈0.05）;bone mineral density of femurs and tibia in DZ group were remarkably increased （P〈0.05）. Conclusion Total extraction in Eu-commia ulmoides oliv. leaf can improve bone metabolism of OP animals,increase BMD,prevent and treat osteoporosis effectively.