中文摘要：

目的研究杜仲叶活性部位Ⅰ对人体骨代谢平衡的多靶位双重调控作用。为补肾壮骨中药临床防治骨质疏松症（OP）提供理论依据。方法从杜仲叶5个极性不同的提取部位（Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ）中选取对骨代谢平衡调节最有效部位Ⅰ；建立体外大鼠成骨细胞培养、肾细胞培养以及两种细胞共培养实验模型，加入高、中、低剂量杜仲叶活性部位Ⅰ培养；生物化学法测定成骨细胞早期特异性分泌的ALP活性，ABC—ELISA法测定肾细胞OPG和成骨细胞OPG与ODF的分泌量；RT—PCR技术检测OPG基因mRNA转录量。结果杜仲叶活性部位Ⅰ促成骨作用表现剂量依赖性。对成骨细胞作用明显的剂量是10^-3g·ml^-1（P〈0．001），对肾细胞作用明显的剂量是10^-4g·ml^-1（P〈0．05）。成骨细胞与肾细胞共培养的最佳比例是2：1。结论杜仲叶活性部位Ⅰ在成骨细胞、肾细胞不同靶部位对人体骨代谢平衡具有双重调控作用。

英文摘要：

Objective To study the dual and muhitarget adjusting metabolism of bone with active component Ⅰ from Duzhongye, provides the theoretical basis for preventing and treating osteoporosis with the Chinese medicine which can tonify the kidney and strengthen bone. Methods To select the most effective component Ⅰ on adjusting metabolism balance of bone from five different polar component （Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ） abstracted from Duzhongye. The experimental models were set up to culture the rat＇s osteoblast, kidney cells and osteoblast-kidney cells coculture, then the active component Ⅰ abstracted from Duzhongye with different concentrations were added to the culture medium. The ALP secreted specifically by osteoblast was tested by biochemical method, the OPG secreted by kidney cells and the OPG and ODF secreted by osteoblast were tested by ABC-ELISA, and the transcription of OPG mRNA was tested by RT-PCR. Results The cells grew especially significant with different concentrations of the component Ⅰ ,10-5 g·ml^-1 affected the osteoblast obviously（P 〈 0.001） and 10-4 g·ml^-1 affected the kidney cells remarkably（ P 〈 0.05 ）. The best ratio of the osteoblast co-cultured with the kidney cells was in 2 ： 1. Conclusion The active component of Duzhongye can dual adjust metabolism balance of bone on the different targets position of osteoblast and the kidney cells.