中文摘要：

目的 探讨骨形成发生蛋白4（BMP4）在人脑胶质瘤细胞株U251中的作用及其信号通路.方法 阿霉素、BMP4质粒体分别给予U251细胞,BMP4的小干扰给予阿霉素处理过48 h的U251细胞,RT-PCR、荧光定量PCR和Western blot检测转染是否成功及BMP4、SMAD4的表达量.MTT检测细胞增殖活性,应用公式（1-处理组OD/空白组OD）×100%计算处理组细胞增长抑制率.结果 与空白组相比,阿霉素组和BMP4转染组,细胞增殖活性降低,BMP4高表达,SMAD4低表达;干扰组与阿霉素组相比,细胞增殖活性升高,BMP4低表达,SMAD4高表达.结论 阿霉素可以抑制U251细胞,BMP4与细胞抑制相关,BMP4通过BMP4-SMAD4信号通路抑制U251细胞,BMP4可能是胶质瘤治疗的新靶点.

英文摘要：

Objective To investigate the effects of bone morhogenetic proteins 4 （BMP4） and the probable signal pathway in human brain glioma cell line U251.Method Adriamycin or plasmid of BMP4 was given to U251 cells and BMP4 siRNA was given to U251 cells which had been given adriamycin for 48 h.MTT was used to detect the activity of U251 cell growth in control group and treatment group,the formula（ 1 -treatment group OD/control group OD） × 100% was used to calculate U251 cells growth inhibition rate. Reverse transcription - PCR （ RT - PCR ）, fluorescence quantitative PCR （ FQ - PCR ） and Western blot were used to observe whether the translation was sucessful as well as the expression of BMP4 and mothers against decapentaplegic homolog 4 （ SMAD4 ）.Results Compared to control group, U251 cells were inhibited when adriamycin or plasmid of BMP4 was given to U251 cells.The expression of BMP4 was up -regulated and SMAD4was down-regulated.Compared to the adriamycin group,the cells inhibition was increased when BMP4 siRNA was given to adriamycin -treated U251 cells for 48 h.The expression of BMP4 was decreased and the SMAD4 was increased.Conclusions Adriamycin could inhibit U251 cells.BMP4 is associated with the inhibition of U251cells through BMP4 - Smad signal pathway.BMP4 may be a new target for glioma therapy.