中文摘要：

目的 探讨赖氨大黄酸（RHL）对胆汁淤积性肝纤维化模型大鼠Smad2、Smad3表达的影响。方法 采用胆总管结扎（BDL）的手术方法建立大鼠胆汁淤积性肝纤维化模型,将大鼠随机分为5组：对照组、模型组、赖氨酸组、低剂量RHL组[35mg/（kg·d）]、高剂量RHL组[70mg/（kg·d）]。应用实时荧光定量PCR的方法检测大鼠肝脏组织中Smad2、Smad3mRNA的相对表达量。Western blot的方法检测Smad2、Smad3蛋白时相对表达量的变化。结果 与模型组大鼠比较,经RHL治疗后胆汁淤积性肝纤维化大鼠肝脏组织中Smad2、Smad3mRNA相对表达量降低,差异有统计学意义（P〈0.05）;与模型组大鼠相比,经RHL治疗后胆汁淤积性肝纤维化大鼠肝脏组织中Smad2、Smad3蛋白的相对表达量降低,差异有统计学意义（P〈0.05）。结论 RHL能够抑制肝纤维化的发展,可能是通过抑制Smad2、Smad3mRNA和蛋白的表达,阻断TGF-β1/Smads信号传导通路实现的。

英文摘要：

Objective To observe the effects of rhein lysine on expression of Smad2 and Smad3 in the model of cholestatic rat with liver fibrosis .Methods The model of cholestatic hepatic fibrosis rats was established by ligation of bile duct .The rats were as-signed to the following five groups ：control group ,model group ,lysine group ,lower RHL dose group[35 mg/（kg ± d）] and higher RHL dose group[70 mg /（kg ± d）] .The expression of Smad2 and Smad3 were detected by RT-qPCR and the changes of Smad2 and Smad3 were detected by Western blot .Results RT-qPCR showed that compared with the model group ,the expressions of mRNA of Smad2 and Smad3 in both lower RHL dose group and higher RHL dose group were significantly higher （P〈 0 .05） .Western blot showed that compared with model group the expressions in both lower RHL dose group and higher RHL dose group were also sig-nificantly higher （P〈 0 .05） .Conclusion RHL can inhibit the activation and proliferation of cholestatic liver fibrosis .And this re-sult may due to the inhibitory effect on conduction of TGF-β1/Smadss signal as well as the protein and mRNA of Smad2 and Smad3 .