设计了一条绿色高效地提取纯化大豆异黄酮,并联产制备大豆分离蛋白和低聚糖的工艺。首先通过单因素实验和响应面(RSM)优化,确定了超声辅助提取大豆异黄酮的工艺条件:超声功率250 W,70%乙醇,提取温度70℃,提取时间120 min,大豆异黄酮提取量为2 104.25μg.g-1,提取率85.34%;并通过三步简单的分离纯化从豆粉中得到1 838.00μg.g-1、纯度为62.09%的异黄酮,最终收率74.54%;最后以提取异黄酮后的剩余豆渣为原料,联产制备了大豆分离蛋白(216.25 mg.g-1)和低聚糖(73.75 mg.g-1),全过程无废液废渣排出。
An effective and green strategy was developed to obtain isoflavones from soybean.Soy protein isolates and oligosaccharides were also obtained from soybean dregs simultaneously.Firstly,the optimal extraction conditions were investigated by single factor experiments and RSM optimization.A high extraction amount(2 104.25 μg·g-1)and yield(85.34%)of isoflavones were achieved under the optimal extraction conditions:ultrasonic power of 250 W,70% ethanol,70℃,120 min.Secondly,the purified isoflavones of 1 838.00 μg·g-1 was obtained with purity of 62.09% and recovery of 74.54% via three simple purification steps.Finally,the amount of soy protein isolates and oligosaccharides obtained from soybean dregs achieved 216.25 mg·g-1 and 73.75 mg·g-1,respectively.There is no waste liquid and solid generated from the whole process.The soybean could be utilized comprehensively through this strategy.