中文摘要：

为了快速准确地测定小麦直、支链淀粉含量，通过单波长法、双波长法、多波长法对比色法测定小麦直、支链淀粉含量进行了比较，并对影响测定结果的试验条件进行了优化和分析。结果表明，与马铃薯相比，小麦面粉直、支链淀粉-碘复合物吸收曲线存在一定的差异。用单波长法、双波长法、多波长法测定小麦直、支链淀粉含量，均能得到相关性很高的回归方程。双波长和多波长法可以有效地排除其他物质的干扰，测定结果优于单波长法。多波长法测定复杂，结果计算繁琐，因此双波长法更适用于小麦直、支链淀粉含量的同时测定，其测定结果也优于多波长法。在双波长法中，小麦直链淀粉的适宜测定波长为630和486nm，小麦支链淀粉的适宜测定波长为550和743nm。溶液酸碱度pH值3．5左右。

英文摘要：

In order to determine amylose and amylopectin content rapidly and accurately and guide genetic improvement of wheat starch, different spectrophotometric methods for determining amylose, amylopectin and total starch content were compared among single, double and six wavelengths. In addition, the factors, such as pH value, affecting the measurement were optimized and discussed. Results showed that the iodine-binding capacity of wheat starch was quite different from that of potato displayed by the absorbance spectrum of complexes formed with iodine. Regression equations with high correlation coefficients were developed from different methods of single, double, and multi wave- lengths, respectively. The methods with both double and six wavelengths were more suitable to deter- mine amylose and amylopectin content simultaneously and rapidly than that with single wavelength be- cause they excluded the interference of other biomoleculars existing in the reaction system efficiently. Furthermore, wheat amylose and amylopectin content determined by double wavelengths was more accurate and simple than by multi wavelengths. Optimized from this experiment, the amylose content was determined by 630 and 486 nm, and amylopectin by 550 and 743 nm, respectively. The pH value of the reaction system was around 3.5.