中文摘要：

为确定灰毛豆Tephrosia purpurea树皮甲醇提取物中的杀虫成分,以白纹伊蚊Aedes albopictus 4龄幼虫为靶标昆虫,在活性跟踪的基础上利用色谱技术分离其活性成分,然后根据各化合物的核磁共振图谱和质谱数据确定化合物的结构,并利用玻片载蚜法和点滴法测定了各化合物对桃蚜Myzus persicae无翅蚜成虫和小菜蛾Pluttella xylostella 3龄幼虫的毒杀活性。结果表明：从该植物树皮甲醇提取物中共分离、鉴定了10个对白纹伊蚊幼虫具有毒杀作用的化合物,即12a-羟基鱼藤酮（12a-hydroxyrotenone）,4’-hydroxyemoroidocarpan,豆薯内酯（pachyrrhizine）,鱼藤酮（rotenone）,6-甲氧基香豆素（6-methoxycoumarin）,（-）-edunol,obovatin,pongachin,12-acetyelliptinol和2-hydroxyrotenone。这些化合物对该蚊虫幼虫处理24 h的LC50值分别是12.5,22.1,25.0,34.1,43.4,58.4,121.9,191.0,219.8和250.0 mg/L。3个化合物（4’-hydroxyemoroidocarpan,鱼藤酮和12a-羟基鱼藤酮）对桃蚜成虫和小菜蛾3龄幼虫表现出毒杀活性,它们对桃蚜24 h的LC50值分别是49.9,1.9和0.9 mg/L,对小菜蛾幼虫24 h的LD50值分别是49.8,197.1和40.9μg/头。首次从该植物中分离得到6个已知的黄酮类化合物[4’-hydroxyemoroidocarpan,2-hydroxyrotenone,（-）-edunol,12-acetyelliptinol,pongachin和obovatin]和2个已知的香豆素类化合物（6-甲氧基香豆素和豆薯内酯）。阐明这些杀虫化合物的结构不仅有利于理解植物和昆虫的关系,而且有助于评价该植物及其活性化合物作为植物源农药开发利用的潜力。

英文摘要：

In order to determine insecticidal compounds from the methanol extracts of Tephrosia purpurea bark,the active compounds were isolated by activity-guided fractionation with column chromatography and identified based on NMR（nuclear magnetic resonance） and MS（mass spectrometry） data.Slidedip method was performed to determine the insecticidal activities of each compound against Myzus persicae adults,and topical application was conducted to determine contact toxicity of each compound against the 3rd instar larvae of Pluttella xylostella.Ten known compounds were isolated and identified,i.e.,12ahydroxyrotenone,4’-hydroxyemoroidocarpan,pachyrrhizine,rotenone,6-methoxycoumarin,（-）-edunol,obovatin,pongachin,12-acetyelliptinol and 2-hydroxyrotenone.All these compounds exhibited insecticidal activity against the 4th instar larvae of Aedes albopictus with the LC50 value being 12.5,22.1,25.0,34.1,43.4,58.4,121.9,191.0,219.8 and 250.0 mg/L,respectively at 24 h after treatment.Moreover,three compounds（4’-hydroxyemoroidocarpan,rotenone and 12a-hydroxyrotenone） exhibited insecticidal activity against M.persicae adults and the 3rd instar larvae of P.xylostella with their corresponding LC50 values being 49.9,1.9 and 0.9 mg/L against M.persicae adults,and with the LD50 values being 49.8,197.1 and 40.9 μg/individual against P.xylostella larvae,respectively.Eight known compounds,i.e.,4’-hydroxyemoroidocarpan,2-hydroxyrotenone,6-methoxycoumarin,pachyrrhizine,（-）-edunol,12-acetyelliptinol,pongachin and obovatin,were isolated from T.purpurea bark for the first time.The elucidation of the structure of these phytochemicals and their insecticidal activity is important not only for understanding the insect-plant relationships,but also for assessing the potential of this plant as botanical insecticide to be explored and utilized.