中文摘要：

[目的]研究灰毛豆叶片甲醇提取物中黄酮类化合物,并测试它们对斜纹夜蛾卵巢细胞（SL细胞）的细胞毒性.[方法]对甲醇提取物的分离采用了多种色谱技术分离纯化,并结合各种光谱分析（包括UV,1D,2D NMR分析以及HR-ESR-MS）进行结构鉴定.细胞毒性的测试采用的是MTT法.[结果]从灰毛豆叶片甲醇提取物中分离得到6个黄酮类化合物：6-methoxykaempferol （1）,6-methoxykaempferol 7-O-α-rhamnopyranoside （2）,6-methoxykaempferol 3-O-α-rhamnopyranosyl（1 →2）[α-rhamnopyranosyl（l→6）]-β-galactopyranoside （3）,6-methoxykaempferol 3-O-α-rhamnopyranosyl （1→2） [α-rhamnopyranosyl（l→6）]-β-galactopyranoside-7-O-α-rhamnopyranoside （4）,pongachin （5）,5,7-dimethoxy-8-（3-hydroxy-3-methylbut-1Z-enyl）flavanone（6）.除了化合物5之外其余化合物均为首次从灰毛豆中分离得到.细胞毒性测试发现pongachin （5）具有明显的细胞毒性,其IC50为4.4 mg/L,化合物1,3和5的细胞毒性均大于阳性对照鱼藤酮.[结论]灰毛豆叶片中6-methoxykaempferol类化合物（1-4）含量相对较大,化合物1,3和5在对SL细胞的活性实验中细胞毒性均大于阳性对照鱼藤酮,值得进一步研究.

英文摘要：

[Objective] The aim was to determine flavonoids from the MeOH extracts of Tephrosia purpurea leaves and their cytotoxicitives against the ovarian cells from Sprodenia litura （SL cells）.[Method] The compounds were isolated with column chromatography and their structures were established on the basis of various spectroscopic analysis （including UV,1D and 2D NMR analyses as well as HR-ESRMS）.The cytotoxicity against the SL cells was evaluated by using MTT method.[Result] Six known flavonoids,6-methoxykaempferol （1）,6-methoxykaempferol 7-O-α-rhamnopyranoside （2）,6-methoxykaempferol 3-O-α-rhamnopyranosyl（1→2）[α-rhamno-pyranosyl（1→6）]-β-galactopyranoside （3）,6-methoxykaempferol 3-O-α-rhamnopyranosyl （1 →2）[α-rhamnopyranosyl （1 →6）]-β-galactopyranoside-7-O-α-rhamnopyranoside （4）,pongachin （5）,5,7-dimethoxy-8-（3-hydroxy-3-methylbut-1Z-enyl） flavanone （6） were isolated and determined.Except compound 5,the others were isolated from T.purpurea for the first time.For the cytotoxicity compound 5 had significant activity with the IC50 value of 4.4 mg/L while compound 1 and 3,whose cytotoxicity exceeded rotenone,also showed moderate activity.[Conclusion] Of all the compounds from T.purpurea leaves,the content of 6-methoxykaempferol compounds was considerable.The profiles of these compounds against SL cells suggested that compounds 1,3 and 5,whose cytotoxicity exceeded rotenone,were worth further research.