中文摘要：

目的探讨孕激素对淋球菌（Ng）诱导的人宫颈癌细胞（Hela）NALP3炎性体基因表达的影响。方法将体外培养的Hela细胞分为5组，即空白对照组、Ng组、Ng＋10^-9mol／L孕酮组、Ng＋10^-8mol／L孕酮组和Ng＋10^-7mol／L孕酮组。荧光定量PCR检测Hela细胞内NALP3mRNA表达，Western Blot检测Hela细胞内NF-κBp65和caspase-1p20活性的变化，酶联免疫吸附试验法（ELISA）测定各组细胞培养上清液中白细胞介素-1β（IL-1β）的含量。结果Hela细胞在淋球菌的刺激下，细胞内NALF3mRNA表达、NF-κB和caspase-1p20活性、细胞培养上清液中的IL-1β含量均明显升高，Ng组与空白对照组比较差异有统计学意义（P〈0．01）；加入孕酮干预后，随着孕酮浓度的增高，细胞内NALV3mRNA表达、NF-κB和caspase-1p20活性、细胞培养上清液中的IL-1β含量明显降低，与Ng组比较，差异均有统计学意义（P均〈0．01）。结论孕酮可抑制淋球菌诱导的NALP3表达及caspase-1的活化和IL-1β的分泌，且这种抑制作用可能是通过抑制NF-κB活性实现的。

英文摘要：

Objective To investigate the regulatory effects of progesterone on NALP3 expression in Hela challenged with Neisseria gonorrhoeae（Ng）. Methods Hela were cultured and divided into five groups randomly： control group, Ng group, Ng ＋ 10^-9 mol/L progesterone group, Ng ＋ 10^- 8 mol/L progesterone group and Ng ＋ 10^-7 mol/ L progesterone group. The mRNA expressions of NALP3 in the cells were determined by real-time PCR. West- ern Blot was preformed to determine the activities of NF-κB and caspase-lp20. The protein of interleukin-1β （IL-1β ） were determined by enzyme- linked immunosorbent assay （ELISA）. Results The mRNA expressions of NALP3,the activities of NF-κB and caspase-lp20 in Hela and the levels of IL-1β in the supernatant in- creased significantly than that in the controls （P 〈 0.01 ）. After adding progesterone into the cells challenged with Neisseria gonorrhoeae ,the mRNA expressions of NALF3 ,the activities of NF-κB and caspase-lp20 in Hela and the levels of IL-1βin the supernatant decreased with the increasing of progesterone concentrations. Con- clusion Progesterone can dose-dependently inhibit the expression of NALP3, the activation of caspase-1 and the production of pro-inflammatory cytokines IL-1β in Hela challenged with Neisseria gonorrhoeae. And the de- creased NALP3 mRNA expression may be associated with the inhibition of NF-κB activity by progesterone.