欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
以海洋来源放线菌无活性野生株HLF-43为出发菌,通过微波诱变结合新霉素抗性筛选,得到新霉素抗性突变株共128株,其中微波诱变新霉素抗性株117株、自发突变抗性株11株。经活性筛选,8株微波诱变新霉素抗性突变株对K562细胞有一定抑制作用,100μg/mL发酵样品对K562细胞的抑制率〉20%。经对不同微波辐照时间和不同浓度新霉素组合筛选实验结果的比较分析,初步确定对HLF-43的微波辐照诱变最佳时间为120 s。
英文摘要：
Total 128 neomycin-resistant（neo） mutants including the 117 mutants from microwave mutagenesis and the 11 mutants from auto-mutagenesis were obtained by neo mutant selection on neomycin-containing plates coupled with microwave-induced mutation technique.Among the neo mutants obtained,the 8 neo mutants from microwave mutagenesis appeared to be bioactive with the inhibition rates over 20% at the 100μg/mL of sample concentration on K562 cells.By combination of the different time of microwave irradiation and the different concentration of neomycin,it has been appeared that the 120 s of microwave irradiation is likely to be favorable for obtaining more neo mutants.
同期刊论文项目
同项目期刊论文
Purpurogemutantin and Purpurogemutantidin, New Drimenyl Cyclohexenone Derivatives Produced by a Muta
Pseudoverticin, a novel benzoquinone- derived ansamycin antibiotic, obtained as new cell cycle inhib
期刊信息
位置：