中文摘要：

以甜菜叶片为材料，用CTAB法提取基因组DNA。以分段PCR法扩增得到了完整的甜菜胞质型谷氨酰胺合成酶（GS1）基因组DNA。采用RT-PCR法扩增此GS1基因（GS1）的cDNA序列应用于对照。获得了长度为9606bp的完整的GS1 DNA序列和长度为1068bp的GS1 cDNA序列。分析GS1基因组DNA序列表日月，它包含13个外显子，被12个内含子分隔开。其外显子区与已公布的GS1 mRNA序列的相似性达99．5％。RT-PCR法获得的cDNA序列与已知的GS1 mRNA序列相似性达99．6％。而2次实验中GS1基因组DNA外显子区与GS1 cDNA序列的相似性达99．9％。GenBank登录号为EU370974。

英文摘要：

Genomic DNA was extracted from sugar beet leaves by CTAB method in this paper. Cytosolic glutamine synthetase （GS1） genomic DNA was cloned by subsection PCR method, and the full-length GS1 gene sequence was successfully obtained. The partial GS1 cDNA sequence was obtained by RT-PCR method and compared to GS1 gene. The full length of GS1 genomic DNA was 9 606 bp, including 13 exons which were separated by 12 introns. The sequence similarity between exon parts of GS1 genomic DNA and the known GS1 mRNA was 99.5%. GS1 cDNA sequence was 1 068 bp, and the sequence similarity between this GS1 cDNA and the known GS1 mRNA was 99.6%. Meanwhile, the sequence similarity between exon parts of GS1 genomic DNA and GS1 cDNA cloned in the experiment was 99.9%. The gene accession number in GenBank was EU370974.