中文摘要：

目的 研究吗啡成瘾大鼠消退期高频脑深部电刺激（DBS）刺激双侧伏隔核对吗啡诱导位置偏爱及复吸行为的影响.方法 选取成年SD大鼠20只,体重280~300 g,将电极外套管通过立体定向手术植入大鼠双侧伏隔核核心部,术后休息5 d,应用腹腔注射吗啡（10 mg/kg）诱导建立吗啡成瘾大鼠位置偏爱模型,建模成功后采用随机数字表法将大鼠随机分为实验组（吗啡＋DBS,n=9）和对照组（吗啡＋假刺激,n=9）,通过DBS电路给予电刺激;实验组给予高频电刺激（刺激参数：频率120 Hz,波宽60 ms,刺激强度0.3 mA,刺激时间30 min/d）,对照组给予假刺激.刺激后次日开始测试两组大鼠位置偏爱评分至消退成功,比较两组消退时间;两组大鼠分别于消退成功24 h内诱导复吸,测试位置偏爱评分并进行组间比较.结果 实验组成瘾消退期平均时间（26 d）较对照组（6 d）明显延长.点燃复吸后,实验组伴药箱停留时间为（357.01±192.72） s,显著短于对照组[（704.91±181.35） s;t=2.370,P=0.034 6].结论 高频刺激成瘾大鼠双侧伏隔核会延长成瘾行为消退时间,但会抑制点燃复吸行为.

英文摘要：

Objective To investigate the influence of deep brain stimulation （DBS） at high frequency to the bilateral nucleus accumbens on morphine-induced conditioned place preference （CPP） and relapse behaviors during extinction phase in rats. Methods Twenty adult SD rats were employed in the experiment. Through stereotactic operation, outer electrode cannula was implanted into rats＇ bilateral nucleus accumbens. After 5 days of rest, the morphine-dependent rat model with CPP was established through intraperitoneal morphine injection （10 mg/kg）. The rats, after being randomly divided into experimental group （morphine＋DBS） and control group （morphine＋sham DBS）, were electrically stimulated using DBS circuits. Rats in the experimental group were given high frequency electrical stimulations while the control group was given sham stimulation. The CPP score of the two groups was recorded the day after stimulation until successful extinction and then the extinction time was compared between the two groups. After successful extinction the rats were given small dose of morphine to trigger relapse within 24 hours, and the CPP score was recorded and compared between the two groups.Results Compared with the control group （six days）, the experimental group （26 days） had a longer extinction time. After relapse, the retention time within the drug-paired chamber of the experimental group was （357.01±192.72） s, obviously shorter than that of the control group （（704.91±181.35） s;t=2.370, P=0.034 6）. Conclusion High frequency DBS to rats＇ bilateral nucleus accumbens can prolong extinction time but inhibit relapse behavior.