中文摘要：

目的：探讨瘦素（leptin）对人脑胶质瘤U87MG细胞迁移及侵袭能力的影响及其机制。方法：Leptin处理U87MG细胞,采用细胞划痕实验检测U87MG细胞的迁移能力,Transwell实验检测U87MG细胞的侵袭能力,RT-PCR及Western blot-ting法检测U87MG细胞中MMP-2及MMP-9 mRNA和蛋白的表达。结果：Leptin明显促进U87MG细胞迁移能力[（152.42±3.29）vs（83.24±2.61）μm,P〈0.05]和侵袭能力[（31.78±5.04）vs（17.03±2.41）个细胞,P〈0.05],leptin能显著上调U87MG细胞中MMP-2、MMP-9 mRNA[（0.76±0.04）vs（0.35±0.02）,（0.84±0.02）vs（0.41±0.06）;均P〈0.05]及蛋白[（0.79±0.03）vs（0.23±0.01）,（0.81±0.05）vs（0.39±0.03）;均P〈0.05]的表达。MMP抑制剂GM6001（10μmol/ml）可以逆转leptin对U87MG细胞迁移[（82.05±2.98）vs（81.76±3.25）μm,P〉0.05]和侵袭能力[（19.23±2.46）vs（18.02±1.98）个细胞,P〉0.05]的影响。结论：Leptin可以促进人脑胶质瘤U87MG细胞的侵袭及迁移,其机制可能与上调MMP-2、MMP-9的表达有关。

英文摘要：

Objective： To investigate the effect of leptin on the migration and invasion of human glioma U87MG cells,and explore its molecule mechanism.Methods： U87MG cells were treated with leptin,the cell migration was analyzed by cell scratch assay,cell invasion was detected by Transwell,and mRNA and protein expressions of MMP-2 and MMP-9 in U87MG cells were observed by RT-PCR and Western blotting assay.Results： Leptin significantly promoted migration（[152.42±3.29] vs [83.24±2.61] μm,P0.05）,and invasion（[31.78±5.04] vs [17.03±2.41]/field,P0.05） of U87MG cells;the mRNA and protein expressions of MMP-2 and MMP-9 were significantly upregulated by leptin in U87MG cells（mRNA： [0.76±0.04] vs [0.35±0.02],[0.84±0.02] vs [0.41±0.06],P0.05;protein： [0.79±0.03] vs [0.23±0.01],[0.81±0.05] vs [0.39±0.03],P0.05）;MMP inhibitor GM6001 reversed U87MG cell migration（[82.05±2.98] vs [81.76±3.25] μm,P0.05] and invasion（[19.23±2.46] vs [18.02±1.98]/field,P0.05） induced by leptin.Conclusion： Leptin can promote the invasion and migration of human glioma U87MG cells,which might related with the upregulation of MMP-2 and MMP-9.