中文摘要：

目的：ERIC—PCR（enterobacterialr epetitive intergenic consensus—PCR）指纹图谱方法研究四君子汤、理中汤、补中益气汤、益胃汤四种中药复方对醋酸胃溃疡脾虚大鼠的治疗作甩。方法：利用食醋灌胃塑造大鼠醋酸胃溃疡脾虚模型，分别提取大鼠健康、模型和复方中药治疗后粪便中的肠道菌群总DNA，并进行ERIC—PCR，建立醋酸模型脾虚大鼠肠道菌群ERIC—PCR指纹图谱，确定其相关特征条带，以各阶段特征条带的净面积和相似性系数为指标，比较四种复方对醋酸胃渍疡脾虚大鼠的治疗作用。结果：益胃汤低中高剂量和四君子汤中剂量治疗后，特征条带500bp净面积显著性降低，相似性系数与模型组相比显著性增加，疗效较好。结论：醋酸模型脾虚主要造成胃溃疡，单纯补气健脾中药治疗效果不明显，益胃汤通过滋养脾胃，对胃溃疡型脾虚有较好治疗效果．

英文摘要：

Objective： To compare the therapeutic effects of four complex prescriptions of TCM （Si-Jun-Zi-Decoction, Li-Zhong-Decoction, Bu-Zhong-Yi-Qi-Decoction and Yi-Wei-Decoction） on the Pi-deficient rats with gastric ulcers using enterobaeterial repetitive intergenic consensus-PCR （ERIC-PCR） fingerprint profiling. Methods： The Pi-deficient rats with gastric ulcers were induced with acetic acid, and profiles of intestinal microflora of Pi-deficient rats with gastric ulcers were established based on ERIC-PCR analysis. According to the band areas and similarity coefficients, the therapeutic effects of four complex prescriptions were compared. Results： Three doses of Yi-Wei-Decoction and middle dose of Si-Jun-Zi-Decoction were demonstrated to produce more recovery activities in Pi-deficient rats, with a significant decrease of 500 bp band and significant increase of similarity coefficients. Conelusions： In the rat model of acetic acid induced gastric ulcer Pi-deficiency, Bu-Qi-Jian-Pi recipes treatment does not exhibit ideal therapeutic effects, while Yi-Wei-Decoction shows much better effects.