中文摘要：

我们在一个人工的湖在浮游生物社区作文评估了季节的变化。我们进行了显微镜的分析和放大 PCR 的部分 16S rRNA 和 18S rRNA 基因的使中毒的坡度胶化电气泳动(DGGE ) 描绘浮游生物社区。与算术平均数(UPGMA ) 聚类未加权的对组方法然后被用来调查这些浮游生物社区的类似。采指纹的 DGGE 表明在一个季节以内在不同地点收集的样品分享了高类似并且通常一起被组织。相反，我们没基于显微镜的分析观察任何季节的变化。在与环境因素的关系的运作的分类单位(OTU ) 揭示了的浮游生物的冗余性分析(RDA ) 透明性否定地与第一轴(R=0.931 ) 被相关，并且温度和全部的磷(TP ) 是，这断然与第一轴相关(R=0.736 和 R=0.660，分别地) 。在结论，在人工的湖的浮游生物社区展出了重要季节的变化。透明性，磷和温度看起来是在浮游生物作文驾驶差别的主要因素。

英文摘要：

We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis （DGGE） of PCR-amplified partial 16S rRNA and 18S rRNA genes to characterize the plankton community. The clustering ofunweighted pair group method with arithmetic mean （UPGMA） was then used to investigate the similarity of these plankton communities. DGGE fingerprinting revealed that samples collected at the different sites within a season shared high similarity and were generally grouped together. In contrast, we did not observe any seasonal variation based on microscopic analysis. Redundancy analysis （RDA） of the plankton operational taxonomic units （OTUs） in relation to environmental factors revealed that transparency was negatively correlated with the first axis （R=-0.931）, and temperature and total phosphorus （TP） were positively correlated with the first axis （R=0.736 and R=0.660, respectively）. In conclusion, plankton communities in the artificial lake exhibited significant seasonal variation. Transparency, phosphorus and temperature appear to be the major factors driving the differences in plankton composition.