中文摘要：

目的探讨hVEGF165及hBMP-7双基因共表达重组腺相关病毒载体对兔激素性股骨头坏死的修复作用。方法应用细菌脂多糖联合甲基强地松龙制备兔激素性股骨头坏死模型。30只经MRI筛选造模成功动物随机分为模型组、髓芯减压组和病毒治疗组。病毒治疗组动物于髓芯减压术后将rAAV-hVEGF165-IRES-hBMP-7病毒载体注入减压区内。选取病毒注射后12周时间点分别行核素骨扫描,股骨头大体观察、HE染色、VEGF和BMP免疫组化染色检测。结果 MRI检测、大体观察以及HE染色发现早期股骨头坏死动物模型建立成功,且病毒治疗组较髓芯减压组及模型组相比股骨头坏死表现明显减轻,VEGF及BMP表达增强,髓腔组织代谢旺盛,组间差别有统计学意义（P〈0.05）。结论重组腺相关病毒载体rAAV-hVEGF165-IRES-hBMP-7通过增加髓腔组织的血运、增强股骨头区骨组织质量提高激素性股骨头坏死区骨修复能力。

英文摘要：

Objective To investigate the effects of recombinant adeno-associated virus vector co-expressing hVEGF165 and hBMP-7 genes in steroids-induced avascular necrosis of femoral head.Methods The rabbit model of femoral head necrosis was constructed by combination use of lipopolysaccharide and methylprednisolone.30 adult rabbits with femoral head necrosis selected by MRI were randomly divided into three groups as follows：model group,core decompression group and virus treatment group.rAAV-hVEGF165-IRES-hBMP-7 virus vector was injected into core decompression region after core decompression operation in the treatment group.The femoral head was examined by radionuclide scan,gross observation,histopathological staining,and immunohistochemistry staining for VEGF and BMP at 12 weeks after virus injection.Results Early animal model of femoral head necrosis was successfully established according to MRI,gross observation and HE staining.Comparing with model group and core decompression group,in virus treatment group,necrosis of femoral head was significantly reduced;VEGF and BMP expressions were increased;and the metabolism of bone marrow tissue was exuberant.The differences of these three groups were statistically significant（P0.05）.ConclusionsThe recombinant adeno-associated virus vector rAAV-hVEGF165-IRES-hBMP7 can improve bone repair capacity in necrosis region of femoral head by increasing the blood supply of cavum medullare tissue and improving the bone quality of femoral head.