中文摘要：

目的：研究细胞周期蛋白B1反义全长cDNA（AS—CLB1）时抗Lewis肺癌细胞（LL/2）体内外增殖作用的影响，为将AS—CLB1联合用于人肺癌等恶性肿瘤的治疗提供实验依据。方法：（0．01nmol／L－0．1μmol／L）处理LL／2亲本（LP），LL／2空载体（LV）及LL／2／AS—CLB1（LA）三种细胞，分别于1h及24h后用MTT比色法评估对各组细胞的杀伤作用。将上述三种细胞分别接种C57BL／6小鼠，成瘤后用（15mg/kg·d）处理各组动物，3d 1次，一共4次，进行成瘤性及动物生存时间观察；流式细胞仪检测各组动物肿瘤组织细胞周期厦凋亡。结果：无论是泰素帝处理1h还是24h，LA细胞存活率较LP、LV两对照均明显降低（P〈0．05）；其中处理1h后，泰素帝80nmol／L组LA细胞存活率低于对照9倍以上，处理24h后，20nmol／L组LA细胞存活率低于对照7倍左右 。LA组小鼠肿瘤体积明显小于对照（P〈0．05），接种后30天，LP组肿瘤体积大小为1981．29±318．56mm^3，LV组为1673．36±297．96mm^3，LA组仅为421．68±30．16mm^3。LA组肿瘤组织中细胞在G1期所占比例为（89．7±0．5）％，凋亡率为（79．5±1．2）％，均较对照明显增加（P〈0．05）。接种后60天，LA组尚有70％的小鼠存活，LP组仅30％，LV组仅40％存活，LA组动物生存时间明显延长（P〈0.01）。结论：AS—CLB1可以明显增强抗LL／2细胞在体内外的增殖作用，此增强效应可能与AS—CLB1诱导细胞发生C1期阻滞厦凋亡，增强了抗肿瘤作用有关。

英文摘要：

Objective： To study the effect of full-length cyclin B1 antisense cDNA（AS-CLB1） on Taxotere in inhibiting proliferation of Lewis lung carcinoma cells （LL/2） in vitro and in vivo and to provide the foundation for the feasibility of treating human hmg cancer and other malignancies with AS-CLB1 combined with Taxotere. Methods： LL/2 parent cells, LL/2/ vector and LL/2/AS-CLB1 transfectants（LP, LV and LA cells） were treated with Taxotere（0.01 nmol/L-0.1 μmol/L） in vit- ro. The cytotoxicity of Taxotere was evaluated by MTT assay types of cells, C57BL/6 mice were treated with Taxotere （15 mg/kg/day） once every three days for four eycles. Cell cycle anti apoptosis in the tumor tissues were determined by flow eytometry. Results： The survival rate of LA cells treated with taxotere was lower than that of the controls （LP and LV cells; P〈0.05）. At Ih after treatment with taxotere （80nmol/L）, the survival rate was （4.56±1.21）% in LA cells, （46.26±3.53）% in LP cells and （44.25±2.21）% in LV cells. At 24h after treatment with 20nmol/L Taxotere, the survival rate was （5.21±1.39）% in LA cells, whereas it was （36.17±3.63）% in LP and （35.15±3.63）% in LV cells. The tumor volume in the LA group was also lower than that of the controls（LP and LV group; P〈0.05）. At 30 days after the inoculation of tumor cells. the tumor vohune was 421.68±30.16mm^3 in the LA group, 1981.29±318.56mm^3 in the LP group and 1673.36±297.96mm^3 in the LV group. Compared with the control groups, the cells in tumor tissues of the LA group showed G1 arrest and increased apoptosis（p〈0.05）. There were （89.7±0.5）% cells in G1 phase and the apoptotic ratio was （79.5±1.2）% in the LA group. Moreover, 70% of the mice in the LA group were still alive at the end of the observation period for the survival studies. In contrast, only 30% of the mice in the LP group and 40% of the mice in the LV group were still alive. The survival rate in the LA group was significantly higher than in