中文摘要：

目的观察胶原诱导性关节炎（CIA）大鼠外周血中T辅助细胞17（Th17）相关细胞因子白细胞介素（IL）-6,IL-17和IL-23水平的变化。方法取40只Wistar雄性大鼠,随机分为正常对照组、CIA模型组,利用牛Ⅱ型胶原建立CIA大鼠模型,造模成功后观察CIA大鼠的关节肿胀程度,免疫后第48天处死大鼠,抽取静脉血,取血清,同时留取大鼠的肿胀的关节,留取滑膜组织。采用酶联免疫吸附试验（ELISA）观察各组对血清中细胞因子IL-6,IL-17和IL-23水平。结果 CIA鼠与正常对照组比较关节肿胀度明显升高。CIA大鼠外周血血清IL-6,IL-17和IL-23水平明显高于正常对照组。2组比较差异均有统计学（P〈0.05）。结论 Th17相关细胞因子IL-6,IL-17和IL-23在CIA鼠的外周血中过度表达,提示Th17型细胞因子在RA的免疫致病机制中可能起重要作用。

英文摘要：

Objective To investigate IL-6,IL-17 and IL-23 levels in blood of collagen-induced arthritis in rats.Methods Forty Wistar male rats were randomly divided into control group and CIA group.Rat arthritis was set up by injecting bovine Ⅱ collagen（BⅡC）.The hind paw volumes was observed and measured.And we evaluated serum IL-6,IL-17 and IL-23 level.Results The degree of swelling of ankle joints in CIA group was increased since the 2th week.The swollen parenchyma of ankle joint was found in CIA group.The level of IL-6,IL-17 and IL-23 were elevated.Conclusion Th17 type cytokines overexpress in serum and PBMC,and Th17 type cytokine may play an important role in the pathogenesis of RA.