中文摘要：

目的：研究0.01%秋水仙碱处理对采后莲雾果实在冷藏期间生理品质、活性氧代谢和能量代谢的影响,探讨秋水仙碱影响采后莲雾的衰老机制。方法：分别用0、0.01%的秋水仙碱溶液浸泡莲雾果实15 min,于4℃贮藏,检测果实品质、活性氧代谢和能量代谢的变化。结果：秋水仙碱处理可以抑制贮藏期间絮状绵软指数的上升,第4天之后效果显著（P〈0.05）,处理后的果实质量损失率整个贮藏期间显著低于对照组（P〈0.05）;秋水仙碱处理可以减缓超氧化物歧化酶（SOD）活性的下降,抑制过氧化氢酶（CAT）活性高峰的出现,保持较低CAT活性,显著提高贮藏后期第8~12天过氧化物酶（POD）活性（P〈0.05）;同时可以保持较高琥珀酸脱氢酶（SDH）和细胞色素C氧化酶（CCO）活性,处理组的SDH活性在第4、8、12天显著高于对照组（P〈0.05）,CCO活性在第2、6、8天显著高于对照组（P〈0.05）;秋水仙碱处理还可以减缓贮藏后期ATP含量和能荷水平的下降,与对照组有显著差异（P〈0.05）。结论：0.01%的秋水仙碱处理能显著延缓采后莲雾果实絮状绵面积的增加;采后莲雾果实絮状绵软面积与能荷水平呈显著负相关性（r=-0.793,P〈0.05）,能量亏缺会加剧莲雾果实衰老;能荷水平与活性氧代谢指标H2O2含量呈显著负相关（r=-0.766,P〈0.05）,与SOD活性呈显著正相关（r=0.802,P〈0.05）,能量代谢会影响活性氧代谢;秋水仙碱主要依靠调节能量代谢起作用。

英文摘要：

Purpose： To investigate the effects of 0.01% colchicine treatment on physiological quality, active oxygen andenergy metabolism of postharvest wax apples during cold storage, and further to understand better the role of colchicine onsenescence of the fruits.Methods： Wax apples were dipped with 0, 0.01% colchicine for 15 minutes at room temperatureand then stored at 4 ℃. The physiological quality, active oxygen metabolism and energy metabolism of wax apples weredetermined during storage. Results： Colchicine treatment could retard the increase of cottony softening extent and had asignificant effect from the 4th day of storage onwards （P 〈 0.05）, the fruits treated with colchicine had a lower percentageweight loss during the whole storage （P 〈 0.05）. Colchicine treatment could also postponed the decrease of superoxidedismutase （SOD） activity during the whole storage, maintained lower catalase （CAT） activity, and significantly increasedperoxidase （POD） activity during the later stage of storage （P 〈 0.05）. At the same time, colchicine treatment could maintainhigher activity of succinate dehydrogenase （SDH） and cytochrome oxidase （CCO）, as compared with the control fruits. TheSDH activity was significantly higher at 4, 8, and 12 day （P 〈 0.05） while the activity of CCO was significantly higher at2, 6 and 8 day （P 〈 0.05）. Also colchicine treatment could slow down the decrease of ATP content and energy charge atlater stage. Conclusions： Colchicine at 0.01% could retard the increase of cottony softening extent of wax apples. Cottonysoftening extent and energy charge had a significantly negative correlation with each other （r = -0.793, P 〈 0.05）. Energydeficit could lead to the senescence of the fruits. Energy charge had a significantly negative correlation with H2O2 content（r = -0.766, P 〈 0.05）, but demonstrated a significantly positive correlation with SOD activity （r = 0.802, P 〈 0.05）. Energy metabolism could influence active oxygen metabol