莲雾果实采后产生的主要病害为软腐病。本试验对莲雾果实采后软腐病病原菌进行了分离纯化,运用形态学和分子生物学对病原菌进行了鉴定,并研究了其病原菌生物学特性。结果表明,引起莲雾果软腐病的病原菌是异色拟盘多毛孢(Pestalotiopsis versicolor)。生物学特性研究结果表明,该病原菌最适生长温度为25℃,最适菌丝生长的相对湿度为75%~80%,湿度对菌丝生长影响不大,菌丝在pH 3~9之间都能生长,pH 5~6生长较好;最适碳源为麦芽糖,甘露醇不利于菌丝生长;最适氮源为蛋白胨,尿素不利于菌丝生长。该菌对于光照较敏感,完全黑暗能促进其菌丝生长。该菌的孢子致死温度为55℃10min。其生物学特性说明了莲雾果实软腐病病原菌对生存环境具有比较强的适应能力。
Soft rot disease is the major disease of posthawest wax apple.A fungus isolated from soft rot disease wax apple was identified as Pestalotiopsis versicolor by morphological analysis and molecular biotechnology,and its pathogenicity and biological characteristics were studied.The results showed that the optimum temperature for mycelial growth was 25 ℃,and optimum relative humidity was 75%-80%,pH ranged from 3-9 was suitable for the fungus while pH 5-6 was the optimum.The optimum carbon source for mycelial growth was maltose,while mannitol was unfavorable.And the optimum nitrogen source was peptone,while carbamide inhibited its growth.The fungus was sensitive to light,complete dark can promote mycelial growth.The lethal temperature for conidium was 55 ℃ for 10 min.Biological characteristics of the pathogen showed that P.versicolor had strong adaptability to living environment.