中文摘要：

目的：克隆和表达钾依赖钠钙交换蛋白-6（SLC24A6）基因，为进一步阐明其与胰岛素分泌的关系奠定基础。方法：利用RT-PCR观察SLC24A6基因在胰岛素瘤和正常胰腺组织中的表达，并克隆SLC24A6基因全长，将SLC24A6基因构建到原核表达载体pEq32a，在大肠杆菌ROSSET菌（DE3）中诱导表达纯化；将SLC24A6基因构建到绿色荧光真核表达载体pEGFP-C3，利用共聚焦显微镜观察SLC24A6在小鼠胰岛素瘤β-TC3细胞中的定位。结果：SLC24A6基因在胰岛素瘤组织表达明显高于正常胰腺；成功获得原核表达纯化蛋白，大小约80kD；重组载体pEGVP-C3-SLC24A6转染后，定位于小鼠胰岛素瘤β-TC3细胞膜上。结论：SLC24A6可能与胰岛素分泌有关，SLC24A6全长原核表达为进一步研究SLC24A6的生物学功能和蛋白质晶体结构奠定了基础，SLC24A6在胰岛素瘤细胞的定位为进一步阐明其与胰岛素分泌的关系提供了理论依据。

英文摘要：

AIM： To done and express the gene of SLC24A6, which provide the basis to illuminate the relationship between the SLC24A6 and insulin release. METHODS： The gene expression of SLC24A6 was analyzed in the imulinoma and normal pancreatic tissues by RT- PCR. The full - length eDNA sequence was subcloned into pET32a vector, and induced expression and purified in ROSSET （DE3） strain. At the same time, the ORF of SLC24A6 was cloned into green fluorescence protein vector pEGFP- C3 to study the location of SLC24A6 in the, mouse insulinoma β-TC3 cells. RESULTS： The mRNA expression of SLC24A6 in the human insulinoma tissue was significantly higher than that in normal pancreatic tissue. The fusion protein of SLC24A6 was a 80kD protein and was purified successfully by prokaryotie vector in ROSSET strain. The localization of SLC24A6 in the mouse imulinoma β-TC3 cells was located in the membrane of the cells. CONCLUSION： SLC24A6 might be related with insulin release. The prokaryotie expression of SLC24A6 provides the basis for the study on biological function and protein structure, and the location of SLC24A6 in the insulinoma cell will throw light on the relationship with imulin release.