中文摘要：

目的：探讨卵裂期胚胎可溶性人类白细胞抗原G（sHLA-G）表达与胚胎发育和种植的关系。方法：收集受精后第2日和第3日的单胚胎培养液300份，应用流式微球技术（CBA）检测其中sHLA-G的含量，并设置阴性对照（不合有胚胎的胚胎培养上清液）。妊娠组和未妊娠组移植胚胎各100枚。A组移植胚胎均为sHLA-G阳性。B组移植胚胎至少1枚为sHLA-G阴性，C组移植胚胎均为sHLA-G阴性。分析单胚胎培养液sHLA-G的表达与临床妊娠率和种植率的关系。结果：①妊娠组第3日胚胎培养液中平均sHLA-G水平明显高于未妊娠组（P〈0．05）。②妊娠组第3日平均sHLA-G水平高于第2日（P〈0．05），但未妊娠组第3日平均sHLA-G水平升高不明显（P〉0．05）。③A组种植率最高，其次是B组，C组最低（P〈0．05）。④优质胚胎（≥6-细胞Ⅱ级）的平均sHLA-G水平妊娠组明显高于未妊娠组（P〈0．05）。结论：胚胎培养液中sHLA-G的表达水平与胚胎的发育潜能及其种植能力间存在正相关关系。若将第3日sHLA-G浓度（尤其是第2日及第3日sHLA-G浓度变化情况）和胚胎形态学参数评估联合作为选择最优移植胚胎的标准，有望为单胚胎移植提供参考数据。

英文摘要：

Objective： To investigate the relationship of soluble human leukocyte antigen-G （sHLA-G） expression with embryo development and implantation by qualitative and quantitative analysis of this molecule in culture medium from single human embryo at different cleavage stages. Methods： The single embryo culture medium on day 2 and day 3 after fertilization was collected, and the values of sHLA-G in the media were detected by the flow cytometry microsphere array （CBA）. The media without embryo kept under the same condition as media with embryos, served as negative controls. A total of 300 copies of culture media from single embryo were detected for the expression of sHLA-G. The patients who received embryo transfer divided into pregnancy and non-pregnancy groups. In each group, a total of 100 embryos cultured in media being checked for the expression of sHLA-G were transferred. Ninty-four patients received embryo transfer and were divided into three groups according to the values of sHLA-G detected in culture media from transferred embryos. Patients with all the transferred embryos cultured in media being tested positive for HLA-G were classified as group A, for those patients with at least one transferred embryo cultured in media being tested negative for HLA-G were classified as group B, and patients with all the transferred embryos cultured in media being tested negative for HLA-G were classified as group C. The relationship betwdefi the expression of sHLA-G in singte-embryo culture mediurm ahd clinical pregnancy rate and implantation rate was analyzed. Results： 1） The mean levels of sHLA-G on day 3 of embryo culture media in pregnancy group were significantly higher than those in non-pregnancy group （P〈0.05）. 2） The mean levels of sHLA-G on day 3 in pregnancy group were higher than those on day 2 （P〈0.05）, but in non- pregnancy group, there were no significant differences of mean sHLA-G levels between day 2 and day 3 （P〉0.05）. 3） The implantation rate of group A was the highest,