中文摘要：

目的：探讨在医院自慰法取精时间长短与精液质量的关系。方法284例采用自慰法取精的男性不育症患者的精液标本，按世界卫生组织（WHO）操作标准常规分析精液，并测量精浆中性α-葡萄糖苷酶（NAG）、锌（Zn）和果糖（FRU）水平。按照取精时间分成3组：A组，取精时间小于或等于10 min；B组，取精时间为大于10∽15 min；C组，取精时间大于15 min。利用统计软件分析自慰取精时间与精液参数间的关系。结果取精时间与精子浓度（r＝-0．20，P＝0．02）、单次射精总精子数量（r＝-0．20，P＝0．04）、前向运动精子总数（r＝-0．40，P＝0．03）、NAG（r＝-0．20，P＝0．01）、FRU（r＝-0．30，P＝0．02）呈负相关，而取精时间长短与患者年龄、禁欲时间、精液量、精子活力及精浆锌无统计学意义（P＞0．05）。此外，A组精子浓度、单次射精总精子数量和前向运动精子总数明显高于B组和 C 组，且和 C 组比较，A 组精浆 FRU 明显增高，差异均有统计学意义（P＜0．05）。结论取精时间与精液质量密切相关，在医院门诊自慰法取精期间产生的应激紧张可能会引起精液质量下降。

英文摘要：

Objective To investigate the association between the length of time-to-ejaculation and the quality of semen obtained by masturbation.Methods Ejaculates from 284 men under infertility assessment were analyzed according to World Health Organi-zation guidelines.Seminal neutralα-glucosidase （NAG）、zinc（Zn）and fructose（FRU）were also measured.All patients were divid-ed into three groups according to the length of time-to-ejaculation,including group A with time-to-ejaculation not more than 10 min, group B with time-to-ejaculation of 11-15 min and group C with time-to-ejaculation more than 15 min.Statistical analysis was per-formed.Results Time-to-ejaculation was negatively correlated with sperm concentration （r= -0.20,P= 0.02）,total sperm count （r= -0.20,P= 0.04）,NAG （r= -0.20,P= 0.01）,total count of progressive motility （r= -0.40,P= 0.03）and fructose （r=-0.30,P= 0.02）,respectively,but not correlated with age,sexual abstinence,semen volume,sperm motility and Zinc.Sperm con-centration,total sperm count and total count of progressive motility in were higher than group B and C （P〈0.05）,and fructose was higher than group C （P〈0.05）.Conclusion Time-to-ejaculation might be associated with semen parameters.Acute stress during semen collection via masturbation at a clinic could infect the quality of semen.