中文摘要：

为了验证Ri36候选基因的功能，利用长片段PCR（long—rangePCR，LR—PCR）技术从Ri36基因的供体品种Q61中扩增了3个候选基因R36-1，R36—2，R36—3，长度分别为5．9kb，9．6kb和16．5kb。电泳回收目的片段，将只36-1和R36—2分别克隆到双元载体pCAMBIA1300．而将只36—3克隆到双元载体pYLTAC27的AscI位点。为了将朋6—3也克隆到具有高效转化效率的双元载体pCAMBIA1300中．首先在不改变pCAMBIA1300基本骨架的前提下．在其多克隆位点增加了AscI酶切位点．获得新载体pCAMBIA1300AscI；然后将克隆在载体pYLTAC27上的R36～3片段切下来。组装到新载体pCAMBI—A1300AscI上。经过酶切鉴定和测序分析，已成功地获得了3个候选基因的重组阳性克隆．为进一步地研究只36基因的功能奠定了基础。

英文摘要：

To identify three candidate genes predicted, long-range PCR （LR-PCR） was employed to amplify each of the candidate genes based on the genomic DNA of the donor cv. Q61. The appropriate products of the P136-1 and Pi36-2 were purified and then ligated into the binary vector of pCAMBIA1300, as well as that of the Pi36-3 ligated into the binary vector of pLYTAC27 and the reconstructed pCAMBIA1300AscI, respectively. The results would aid to dissect the function of the resistance gene Pi36.