中文摘要：

为了探索链球菌制剂对NOD鼠脾细胞及血清细胞因子的影响，试验以NOD鼠为Ⅰ型糖尿病动物模型，在5～20周龄，皮下注射链球菌制剂，每只0．1mg，对照组注射0．1mL生理盐水，分别取未发病鼠、已发病鼠及经链球菌制剂免疫后小鼠的眼底血、脾脏制成脾细胞DMEM悬液，夹心ELISA法测IFN-1、IL-4的含量．结果发病鼠IFN-γ的分泌量高于链球菌制剂的免疫鼠，而IL-4低于后者。链球菌制剂可能通过调节Th1和Th2型细胞因子的平衡来预防性地治疗糖尿病．从而发挥免疫调节的作用。

英文摘要：

In order to explore the effect of streptozotoein on cytokines in splenocytes and serum of NOD mice, the effectiveness of streptozotoein on diabetes mellitus in mice model was studied. Female NOD mice of streptozotoein-treated group were injected with 0.1mg streptozotoein weekly from 5wk to 20wk and the control group with 0.1mL NS （Normal saline）. Serum IL-4 and IFN-γ were measured by ELISA. IFN-γ in splenocytes of streptozotoein group were significantly downregulated and IL-4 were upregulated compared with control group. Streptozotoein possessed the therapeutic and preventive effects on diabetic mice, which might be related to regualating Th1 and Th2 cytokines balance. Streptozotoein appeared to have an immune-regulatory effect on diabetes mice.