中文摘要：

目的探讨内源性IL-10在脊髓损伤SD大鼠脊髓神经组织中表达的动态变化及其意义。方法采用成年SD大鼠脊髓横断损伤（SCI）模型,经ELISA方法检测脊髓损伤急性期内IL-10在脊髓神经组织中表达的动态变化。采用免疫荧光双标记技术,于荧光显微镜下观察IL-10在脊髓神经细胞和胶质细胞内的表达,及其与神经丝蛋白（NF）和神经胶质细胞标志蛋白（GFAP）的共定位情况。结果①免疫荧光结果显示：正常对照组IL-10在脊髓神经细胞和胶质细胞表达较弱;脊髓损伤后IL-10在脊髓神经细胞和胶质细胞的表达明显增强,且中央管的室管膜细胞亦有较强表达;②定量检测结果表明：IL-10在损伤大鼠脊髓中的表达,从脊髓损伤后第3天呈上升趋势,至第5天出现最大峰值,至第7天有所下降,14 d接近正常水平。结论在脊髓损伤急性期内,脊髓神经细胞和胶质细胞均可表达大量的炎症抑制因子IL-10,根据其动态变化规律,笔者推测IL-10表达上调可能与中枢神经系统内固有免疫反应相关,IL-10可抑制炎症因子的过度表达,从而保护中枢神经系统免受继发性炎症反应造成的损伤。

英文摘要：

Objective To explore the local inflammatory response of neurons and glias in innate immunity following spinal cord injury （SCI）. Methods In this study, with double-label immunofluoreseence method, we observed, by confocal microscopy, the distrbutions of interleukin-10 （IL-10） and IL-10 receptor in the neurons or glias labeled by NF or GFAP. With ELISA, we detected dynamic of IL-10 following spinal cord injury. Results ① IL-10 significantly were expressed in glias, as well as in neurons following spinal cord injury. ②The expression level increased with time-lapse, and the peak showed at the fifth day after injury, and the expression level began to decrease at the seventh day. Conclusions We suggested that neurons, in addition to glias, might initiate the innate immunity and play certain role in CNS immunoreactions after SCI. Moreover, we assumed that the stimulations from might directly activated the neurons and glias, and resulted in that increase of IL-10 could regulate the inflammation such as TNF, IL-1 and IL-6.