中文摘要：

观察纤维蛋白-纤粘连蛋白-层粘连蛋白复合支架移植对大鼠脊髓损伤后神经再生和胶质瘢痕形成的影响，评价该支架移植修复脊髓损伤的可行性。本研究将圆柱状复合支架移植人大鼠脊髓完全横断缺损部位，同时以该模型动物作为对照组。分别于术后4w、8w、12w对动物下肢运动功能进行BBB评分。然后取出支架／脊髓组织，用免疫荧光双标和免疫印迹技术分析损伤部位神经纤维再生和胶质细胞增生情况；并用透射电镜观察支架移植部位的组织学结构。结果显示：术后4～12w支架移植组动物BBB评分明显高于对照组（P〈0．05）。于术后4w，移植组可见支架内有少量神经纤维，此后神经纤维逐渐增多，而胶质细胞增生不明显；对照组脊髓损伤局部可见坏死空洞，空洞周边胶质细胞增生明显。免疫印迹检测结果表明，移植组神经丝蛋白（NF-200）相对含量高于对照组；对照组胶质纤维酸性蛋白（GFAP）的相对含量高于实验组。以上结果提示：纤维蛋白支架移植可促进大鼠脊髓损伤后神经再生，并抑制胶质瘢痕形成。纤维蛋白（原）作为生物材料用于构建修复脊髓损伤的组织工程支架具有良好的应用前景。

英文摘要：

To observe the effect of fibrin based composite scaffold transplantation on the regeneration of nerve fiber and the formation of glial scar after spinal cord injury in rats. In order to appraise the feasibility of this scaffold to repair the injured spinal cord. The rats were randomly divided into two groups： scaffold transplantation group and spinal cord injury control group. Following complete spinal cord transection in both groups, the cylindrical scaffolds composed of fibrin, fibronectin and laminin were implanted only into the injured spinal cord of the scaffold transplantation group. The rats of each group were respectively estimated by the BBB locomotion score system in 4 weeks, 8 weeks and 12 weeks after operation ,and then to be sacrificed. The injured spinal cord segments with or without the scaffolds were removed out and examed. The regeneration of the nerve fibers and the proliferation of the gliacytes were analysed with immunofluorescence and immunoblotting methods. The tissue reconstruction of the scaffold in the injured spinal cord was observed with electron microscop. The results showed from 4 weeks to 12 weeks the BBB locomotion scores of scaffold transplantation group were improved significantly comparing with that of the control group （ P 〈 0. 05 ）. From 4 weeks after operation, the regenerated nerve fibers could grow into the scaffold then became more and more in case of scaffold transplantation, but there were fewer gliocytes in the scaffold. In the control group, the necrotic cavities were formed after spinal cord injury. There were a lot of gliacytes around the cavity. The results of Western blotting showed that the content of the neurofilament （NF） was higher than the glial fibrilliary acidic protein （GFAP） in the scaffold transplantation group. However, the results of the control group were on the contrary. These results suggest that after transplanting scaffold into the injured spinal cord, the fibrin based composite scaffold can promote the regeneration and extension of