中文摘要：

研究了过渡金属铜修饰的仲钨酸盐化合物[Na_2（H_2O）（_10）][Cu_4（H_2O）（_12）（H_2W（_12）O（_42））]·15H2O（Na_2Cu_4W（_12））的体外抗肿瘤活性.取人宫颈癌细胞Hela加入不同浓度Na2Cu4W（_12）处理,应用MTT法分析细胞增殖,计算了半数抑制浓度（IC（_50））;采用光学显微镜观察Hela细胞的凋亡形态变化;用流式细胞术分析细胞周期和细胞凋亡,计算了各期细胞比例及细胞凋亡率.结果表明：Na_2Cu_4W（_12）对Hela细胞增殖抑制的IC（50）值为10.2μmol/L,且呈剂量依赖性;光学显微镜下可观察到Na_2Cu_4W（_12）处理组细胞数目明显减少,出现变圆、缩小、老化等形态变化;不同浓度Na_2Cu_4W（_12）处理12h后,早期凋亡细胞所占的百分比显著增加且呈剂量依赖性.

英文摘要：

The in vitro anti-tumor activity of a novel paradodecatungstate-B compound decorated by transition metal copper：[Na2（H2O）（10）][Cu4（H2O）（12）（H2W（12）O（42））]·15H2O（abbreviated form：Na2Cu4W（12））has been studied.Hela cells were treated with different concentration of Na2Cu4W（12）,then its IC（50） was measured by MTT assay;The morphological change in Hela cells were observed by optical microscopes;Flow cytometry was applied to analyze cell cycle and apoptosis by computing the ratio of Hela cells in different phase and its apoptotic ratio.The results show that Na2Cu4W（12） can inhibit Hela cell proliferation with IC（50） of 10.2μmol/L,showing dose-dependent.Under the optical microscopes,The number of Hela cells treated with Na2Cu4W（12） decreased significantly,cell rounding,shrinking and aging were observed.Treated with different concentrations of Na2Cu4W（12） after 12h,the proportions of Hela cells on the early apoptosis increased markedly,showing dose-dependent.