中文摘要：

目的建立采用UPLC同时测定乐脉颗粒中主要成分没食子酸、丹参素、原儿茶醛、绿原酸、羟基红花黄色素A、芍药苷、阿魏酸、迷迭香酸、紫草酸、丹酚酸B、丹酚酸A的方法。方法采用UPLC色谱系统,色谱柱为BEH C18柱（50mm×2.1 mm,1.7μm）;以0.5%甲酸水溶液（A）-乙腈（B）为流动相,梯度洗脱：0～12 min,97%～73%A;12～13 min,73%～5%A;体积流量为0.4 mL/min;检测波长：芍药苷为230 nm,没食子酸、丹参素、原儿茶醛、丹酚酸B、丹酚酸A为280 nm,绿原酸、阿魏酸、迷迭香酸、紫草酸为324 nm,羟基红花黄色素A为400 nm。结果本方法可在12.5 min内完成一次色谱分析,11种成分的色谱峰均有良好的分离度,方法精密度、重复性的RSD均小于2.0%,各成分均有较宽的线性范围和良好的线性关系（r≥0.999 6）;回收率97.2%～102.7%,RSD 0.50%～1.42%。结论本方法快捷、准确、重复性好,能同时测定乐脉颗粒中11种主要成分,可较全面地控制乐脉颗粒的质量。

英文摘要：

Objective To establish a UPLC method for the simultaneous determination of eleven compounds, gallic acid, tanshinol, protocatechuic aldehyde, chlorogenic acid, hydroxysafflor yellow A, paeoniflorin, ferulic acid, rosmarinic acid, lithospermic acid, salvianolic acid B, and salvianolic acid A in Lemai Granules. Methods Eleven compounds in Lemai Granules were simultaneously determined by UPLC with BEH C18 column （50 mm × 2.1 ram, 1.7 μm）. The mobile phase consisted of 0.5% methanoic acid solution （A）-acetonitrile （B） with gradient elution： 0-- 12 min, 97%--73% A; 12-- 13 rain, 73%--5% A; at the flow rate of 0.4 mL/min, the determination wavelength was paeoniflorin at 230 ran; gallic acid, tanshinol, protocatechuic aldehyde, salvianolic acid B, and salvianolic acid A at 280 rim; chlorogenic acid, ferulic acid, rosmarinic acid, and lithospermic acid at 324 nm; hydroxysafflor yellow A at 400 nm. Results Eleven components were separated clearly and respectively within 12.5 min. The RSD values of precision and reproducibility were all less than 2.0%. The linear relationship between the concentration and peak areas of the eleven compounds was all linear （r 〉 0.999 6）. The average recoveries were 97.2%-- 102.7% with RSD of 0.50%-- 1.42%. Conclusion The method is rapid, simple, reliable, and accurate, and could be used to simultaneously determine the eleven compounds above mentioned in Lemai Granules and for the comprehensive quality control of Lemai Granules.