中文摘要：

目的观察活血解毒方对糖尿病大鼠视网膜中Hippo信号通路的影响。方法选用健康SD大鼠24只,禁食12 h,一次性腹腔注射链脲佐菌素（65 mg/kg）,诱导糖尿病模型。将造模20周后的大鼠随机分为模型组和活血解毒方组。另选取10只大鼠作为正常对照组,给予等体积蒸馏水灌胃。每组每天干预1次,共12周。分别采用免疫组织化学方法、蛋白质印迹（Western blot）方法检测各组视网膜中磷酸化哺乳动物不育系20样激酶（P-MST）、大肿瘤抑制基因1（Lats1）、磷酸化Yes-相关蛋白（P-YAP）、转录相关因子（TAZ）、TEA结构域转录因子（TEAD）蛋白表达水平的变化。结果免疫组织化学方法的结果显示：与正常组比较,模型组大鼠视网膜中P-MST及P-YAP蛋白表达水平降低;与模型组比较,活血解毒方组大鼠视网膜中P-MST及P-YAP蛋白表达水平升高。Western blot的结果显示：与正常组比较,模型组大鼠视网膜中Lats1、TAZ及TEAD蛋白表达水平升高;与模型组比较,活血解毒方组大鼠视网膜中Lats1、TAZ及TEAD蛋白表达水平降低。结论活血解毒方可能通过Hippo信号通路改善糖尿病视网膜病变。

英文摘要：

Objective To observe the effect of Huoxue Jiedu Recipe （HJR）, a Chinese medicine compound, on Hippo signaling pathway in diabetic rat retina. Methods A total 24 healthy SD rats were fasted for 12 h and administered intraperitoneally streptozotocin （65 mg/kg） once to induce diabetic model. After 20 weeks, the diabetic rats were randomly divided into model group and H JR group. And 10 more healthy rats were assigned into normal group with administration intragastrical saline. Each group was in- tervened once daily for consecutive 12 weeks. The levels of phosphorylated mammalian sterile 20-like ki- nase（ P-MST）, large tumor suppressor gene 1 （Latsl）, phosphorylated Yes-associated protein（ P-YAP）, tafazzin （TAZ）, TEA domain family member（TEAD） protein in retinal tissue were detected by immunohistochemical method and Western blot. Results Compared with the normal group, the protein expression of P-MST and P-YAP were decreased in the model group. While compared with the model group, the protein expression of P-MST and P-YAP significantly increased in HJR group. And Western blot results showed that protein expression of Latsl, TAZ and TEAD in model group were higher than those of normal group, and HJR decreased the protein expression of Latsl, TAZ and TEAD. Conclusion The effect of Huoxue Jiedu Recipe on diabetic retinopathy may be via regulating Hippo signaling pathway.