中文摘要：

针对五味子乙醇提取物的复杂体系,借助于复杂样品分析系统软件（CSASS）,根据组分在4次简单线性梯度下42个峰的保留时间,快速准确地计算出各组分的液相色谱保留参数a,c值和峰形参数σ,W1/2。借助这些参数,对五味子色谱的分离情况进行高精度仿真预测。在此基础上,应用移动重叠分辨分离图和谱图仿真技术,发展了计算机辅助的五味子提取物的高效液相色谱全局优化方法。在优化条件下,五味子提取物的高效液相色谱分析可在40min内完成,且常量成分和部分低含量成分都能够得到较好的分离。所建立的方法已成功地用于五味子中化合物保留时间及峰形的预测,并在此基础上对其色谱分离条件进行优化。

英文摘要：

For the separation of the complex system of alcohol fraction of Fructus schisandrae chinensis, complex sample analysis system software （CSASS） was utilized to optimize the high performance liquid chromatographic （HPLC） conditions. The HPLC retention parameters a and c, and the peak shape parameters σ and W1/2 were obtained accurately and rapidly by analyzing 4 linear gradient elutions. Based on the 4 parameters, the chromatogram in any gradient condition could be simulated and predicted precisely. The HPLC optimization method was developed using moved overlapping separation ranging map and simulated chromatogram technology. The HPLC analysis of the alcohol fraction of Fructus schisandrae chinensis could be finished in 40 min, and good resolution for components with general or low abundance was achieved. This method could be used to predict retention times and peak shapes of components in Fructus schisandrae chinensis. And based on the precise predicted results, the optimized separation conditions can be obtained.