中文摘要：

目的：观察小泛素相关修饰物（SUMO）在体外正常培养的人晶状体上皮细胞系（SRA01/04）内的表达以及由高糖诱导的氧化应激条件下SUMO的变化，探讨其对氧化应激的调控作用。方法通过免疫细胞化学染色法，检测SUMO1，2/3，4蛋白在正常培养的SRA01/04内的表达及分布；通过RT-PCR法，观察不同浓度和时间高糖处理时SUMO 1-4 mRNA的表达变化。按浓度实验分组：葡萄糖浓度分别为5.5、12.5、25、50 mmol/L作用24 h。按时间实验分组：葡萄糖浓度50 mmol/L作用0、6、12、24 h。将GFP-SUMO2质粒转入细胞后通过CCK8检测法和AV/PI双染流式细胞仪检测高糖（50 mmol/L）处理后，转染与非转染组的细胞存活率和细胞凋亡率。结果免疫细胞化学染色结果，SUMO 1-4蛋白在SRA01/04细胞内主要分布于细胞核，SUMO 2/3同时少量分布于胞质；RT-PCR结果可见，与低糖组比较，高糖组随着糖浓度增加SUMO1-SUMO4 mRNA表达增加（P〈0.05）；与50 mmol/L高糖处理0 h比较，随着处理时间增加SUMO1-SUMO4 mRNA表达增加（P〈0.05）。与非转染组比较，转染GFP-SUMO2的SRA01/04经高糖处理后存活率增加，凋亡率降低（P〈0.05）。结论 SUMO蛋白在SRA01/04内阳性表达，一定程度高糖诱导的氧化应激影响SUMO mRNA表达。

英文摘要：

Objective To observe the expression of small ubiquitin-related modifiers（SUMO）protein in normal cultured human lens epithelial cells（SRA01/04）and discuss regulation effects of SUMO protein on oxidative stress induced by high glucose. Methods The expression and local-ization of SUMO 1,2/3,4 was detected in normal cultured SRA01/04 cells through immunocytochemistry. The mRNA expression levels of SUMO 1-4 were examined by RT-PCR after the SRA01/04 cells treated with high glucose media at different concentrations and time points. Samples were grouped by medium concentrations（glucoses 5.5 mmol/L,12.5 mmol/L,25 mmol/L,50 mmol/L respectively for 24 h）and by treatment time（0 h, 6 h,12 h and 24 h respectively）. After highly efficient transfection of GFP-SUMO2 into SRA01/04 cells,the survival and apoptotic rates of transfect-ed and un-transfected cells treated with high glucose was detected by CCK8 method and AV/PI double staining flow cytometry. Results The immu-nocytochemistry results showed that SUMO1,2/3,4 proteins were mainly located in the nucleus of SRA01/04 cells and part of SUMO2/3 was in the cytoplasm. RT-PCR results showed that compared with the low-glucose group,the mRNA expression of SUMO1-4 was increased along the increas-ing glucose concentration in the high-glucose group（P〈0.05）. Compared with 0 h,the mRNA expression of SUMO1-4 was enhanced at 6 h,12 h and 24 h（P〈0.05）in the high-glucose group treated at 50 mmol/L concentration. Compared with the un-transfected cells,the survival rate was in-creased and the apoptotic rate was decreased in GFP-SUMO2 transfected cells in oxidative stress induced by high glucose（P〈0.05）. Conclusion SUMO protein was positively expressed in SRA01/04 cells and the expression of SUMO mRNA was affected by oxidative stress induced by high glu-cose.