中文摘要：

目的：研究在高糖诱导人晶状体上皮细胞向间充质转化的过程中JAK-STAT通路的作用及AG490对此过程的影响。 方法：低糖（5.5 mmol/L ） DMEM 培养液体外培养人晶状体上皮细胞系 SRA01/04。高糖（30.5 mmol/L ）处理细胞,按照是否加入AG490及其浓度的不同分为实验组和对照组。 CCK-8试剂盒检测晶状体上皮细胞的活性,选择实验组AG490浓度为10μmol/L和50μmol/L,处理时间分别为6,12,24和48 h；细胞划痕实验检测细胞迁移能力的变化；RT-PCR方法半定量检测TGF-β1,FN和α-SMA的mRNA表达量变化。 结果：在不同的处理时间（6,12,24,48 h ）,高糖组（ HG组）与低糖组相比,细胞的迁移能力增加（P〈0.05）,TGF-β1, FN和α-SMA表达量增高（ P〈0.05）；AG490组与HG组相比,细胞的迁移能力降低（P〈0.05）,TGF-β1,FN和α-SMA的mRNA表达量下降。 结论：JAK-STAT通路通过影响TGF-β1和细胞外基质转录表达参与了高糖诱导的人晶状体上皮细胞向间充质转化的过程。 JAK抑制剂AG490对此过程有抑制作用,且随着AG490浓度的增加其抑制作用增强。

英文摘要：

AlM：To study the relationship between JAK-STAT pathway and epithelial - mesenchymal transition in human lens epithelial cells. Meanwhile, the function of AG490 as a JAK inhibitor was also demonstrated in this article. METHODS：Human lens epithelial cells SRA01/04 （ LECs ） were treated by low concentration of glucose （5. 5mmol/L）. High concentration of glucose （30. 5mmol/L） was used to treat the cells in order to form the high glucose model. According to adding AG490 or not, cells were divided into the control group and the experimental group, appropriate concentration 10＆#252; mol/L and 50＆#252; mol/L of AG490 were chosen and acting time of 6, 12, 24, 48h were selected. Effect of AG490 on cell migration was measured by wound healing test. The expression of TGF-β1 , FN,α-SMA mRNA were examined by RT-PCR. RESULTS：With the prolonged acting time （ 6, 12, 24 and 48h）, cell activity increased in the HG group, as well as more expression of TGF-β1 , FN,α-SMA mRNA were detected compared to the LG group （P〈0. 05）. ln AG490 group, the cell migration activity and expression of TGF-β1 , FN,α-SMA mRNA decreased compared to the HG group （P〈0. 05）. CONCLUSlON：JAK-STAT pathway takes part in high glucose-induced epithelial-mesenchymal transition in human lens epithelial cells. The mechanism is that it impacts the transcriptional expression of TGF- β1 and extracellular matrix. AG490, a JAK inhibitor, inhibits high glucose-induced epithelial-mesenchymal transition in human lens epithelial cells, And the inhibition enhances with the increasing concentration of AG490.