中文摘要：

目的确认骨形态发生蛋白9（Bone Morphogenetic Proteins 9,BMP9）是否促进间充质干细胞C3H10T1/2定向成骨分化,并初步探讨其分子机制。方法用重组鼠BMP9蛋白处理C3H10T1/2细胞。化学发光法检测碱性磷酸酶（ALP）活性;免疫组化检测骨桥素（OPN）的表达;茜素红S染色检测细胞钙盐沉积;Western Blot检测转录因子Smad1/5/8的磷酸化情况。结果 BMP9可以诱导C3H10T1/2细胞早期成骨指标ALP的活性,也可促进晚期成骨指标OPN的表达和钙盐沉积;BMP9刺激后,C3H10T1/2细胞中转录因子Smad1/5/8的磷酸化水平增加。结论 BMP9可以促进间充质干细胞C3H10T1/2定向成骨分化。

英文摘要：

Objective To validate the promotive effect of BMP9 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells,and to study the molecular mechanism underlying BMP9 induced osteogenic differentiation of C3H10T1/2 cells.Methods C3H10T1/2 cells were treated by mouse BMP9 recombinant protein,then the activity of alkaline phosphatase（ALP）was measured by chemiluminescence assay,osteopotin（OPN） expression was detected by immunohistochemisty staining,calcium deposition was detected by Alizerin Red S staining,and activation of Smad1/5/8 was analyzed by Western Blotting.Results BMP9 induced activity of early osteogenic marker ALP promoted OPN expression and calcium deposition of C3H10T1/2 mesenchymal stem cells.Furthermore,BMP9 increased the level of phosphorylated form of Smad1/5/8.Conclusion BMP9 can promote osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.