本研究以远杂9102×徐州68-4杂交后代衍生的重组自交系(RIL)的188个家系为材料,连续3年种植后检测其含油量及脂肪酸含量。结果表明,该RIL群体的含油量及脂肪酸变异丰富,从中获得了含油量稳定高于高值亲本的后代材料1份,油酸含量稳定高于高值亲本的材料23份。RIL群体的含油量、油酸和亚油酸含量以及油酸/亚油酸比的广义遗传力分别为0.849、0.761、0.874和0.887,表明这些性状的变异主要受基因型控制。利用前期构建的SSR遗传连锁图,结合3年主要品质性状鉴定数据,共检测到82个相关QTL,分布在11个连锁群上,其中与含油量、油酸、亚油酸和油酸/亚油酸比(油亚比)相关的QTL分别为15、21、21和25个,贡献率大于10%的主效QTL有23个,2年能重复检测到QTL有8个,3年重复检测到的有4个。其中,本研究新鉴定出的主效QTL有7个,重复性好的有5个,尤其是LG2上区间GM2839-GNB159,3年均定位到与油酸和油亚比相关的QTL,2年定位到与亚油酸相关的QTL,贡献率为5.80%-28.14%,该区间只有1.63c M。这些QTL的获得对于花生品质性状改良中亲本选配、后代标记辅助选择以及QTL精细定位具有重要意义。
Peanut is one of the important commercial and oil crops in the world,and it is one of the key sources of edible vegetable oils. High oil content and reasonable fatty acid composition are the main objects of genetic improvement of peanut quality. In this study,188 recombinant inbred lines( RIL),derived from a cross between two Spanish type peanut cultivars( Yuanza 9102 × Xuzhou 68- 4),and their parents were planted in Wuhan with two replications in three consecutive years. The content of oil,oleic acid,and linoleic acid were measured. The RIL population showed abundant phenotypic variation. Compared with their parents,one line had higher oil content and 23 lines had higher oleic acid content. Genetic analysis showed that the broad sense heritability of oil content,oleic acid,linoleic acid and O/L were 0. 849,0. 761,0. 874 and 0. 887,respectively,which indicated that genetic factors were predominant in determining the phenotypes of these traits. 82 QTLs,distributed on 11 linkage groups,were identified for the four traits,including 15 QTLs for oil content,21 QTLs for Oleic acid,21 QTLs for linoleic acid and 25 QTLs for O / L. Each QTL explained 3. 71%- 28. 14% of the phenotypic variation.There were 23 major QTLs( R^2 10%),8 QTLs identified in 2 years repeatedly,and 4 QTLs were identified in 3years repeatedly. Among them,7 major QTLs and 5 repeatable QTLs were first reported,especially,the marker interval GM2839- GNB159 on LG02,were identified for the 3 traits,including oleic acid and O/L in 3 years repeatedly,linoleic acid in 2 years repeatedly,each QTL explained 5. 80- 28. 14% of the phenotypic variation,which would have great significance for marker- assisted breeding of high quality peanut.