中文摘要：

以分离自中国北部湾沉积物的海洋菌株巴利阿里假单胞菌（Pseudomonasbalearica）为例，在考察其生长动力学的同时，监测了其中心碳代谢（CCM）中6种关键氧化还原酶，葡萄糖-6-磷酸脱氢酶、丙酮酸脱氢酶、2-酮戊二酸脱氢酶、苹果酸脱氢酶、苹果酸酶及异柠檬酸脱氢酶的酶活性时变，从酶蛋白表达水平揭示了海洋生长条件下巴利阿里假单胞菌CCM中关键氧化还原酶的酶活性变化的规律，为研究微生物CCM和发掘海洋微生物新型酶制剂提供一种新思路．

英文摘要：

Assaying the central carbon metabolism （CCM） through the enzyme＇s level may be one effective method to reveal the in teraction between the adaptability of marine bacteria and the extreme environmental conditions. Marine bacteria may have different CCM with terrestrial bacteria as they have different growth environment. So it is valuable to research the related key oxidoreducta ses,which is important to uncover the CCM of marine bacteria. The response of central carbon metabolism of marine bacteria to incu bation time was investigated based on key oxidoreductases detecting,while traditional metabolic analysis was carried out by ~C meta bolic flux analysis. For example,Pseudomonas balearica isolated from sediment of China＇s offshore was investigated. Besides growth kinetics,the central carbon metabolism of P. balearica was uncovered by a comprehensive analysis of six key oxidoreductases, glu cose 6 phosphate dehydrogenase, pyruvate dehydrogenase, 2-ketoglutarate dehydrogenase, malate dehydrogenase, malic enzyme and isocitrate dehydrogenase. The changing regularity of key oxidoreductases activities of central carbon metabolism in P. balearica was revealed on protein level. A new technique for the study of microbial central carbon metabolism and the excavation of new enzyme preparations from marine microbial was provided.