中文摘要：

目的克隆含开放阅读框（ORF）的日本血吸虫SJCHGC08304全基因序列，分析此基因编码蛋白的Wnt受体种类，了解该基因在血吸虫不同发育阶段的mRNA表达差异。方法采用RACE技术从日本血吸虫7d肝期童虫和42d成虫组织中扩增获得日本血吸虫SJCHGG08304全基因序列，通过生物信息学工具分析该基因的完整ORF；同源性分析该基因编码蛋白的种类和功能；运用荧光定量PCR技术分析该基因在日本血吸虫不同发育阶段虫体中的表达情况。结果获得日本血吸虫新基因，该基因ORF含2604bp，编码867个氨基酸，理论分子量为98．264kD。实时定量分析该基因在18d童虫表达量最高，其他依次为14d童虫、42d雄虫、32d成虫、虫卵、23d成虫、27d成虫，而在42d雌虫中没有检测到该基因。结论该基因的氨基酸序列具有典型Wnt受体家族蛋白特征，生物信息学方法同源性分析表明与非洲爪蟾Fz5蛋白的同源性最高，通过比较分析推测为编码日本血吸虫Wnt受体Fz5蛋白基因，命名该基因为sjFz5基因（GenBank登录号：EU370926），其在血吸虫的不同发育时期中有不同程度的差异表达。

英文摘要：

Objective To clone full length gene sequence containing open reading frame （ORF） of Schistosoma japonicum SJCHGCD8304, analyse its category encoding Wnt protein, and to understand the mRNA expression dynamics of the gene during different developmental stages. Methods Based on a EST identified in Genhank, the full length gene of SJCHGC08304 was cloned with RACE technique from 7 d hepatic schistosomula and 42 d adult worms, and its complete ORF was analysed by bioinformatics tools. The expression of the gene during different developmental stages was analysed by using Real-time fluorescent quantitative PCR technique. Results A new gene was obtained, containing a complete ORF with 2 604 nucleotides, and encoding 867 amino acid with 98. 264 kD of molecular mass. Real-time fluorescent quantitative PCR showed that the mRNA level of this gene was the highest in the 18 d schistosomula, followed by 14 d schistosomula, 42 d male worms, 32 d adult worms, egg, 23 d adult worms, 27 d adult worms, respectively, and there were no expression of the gene in 42 d female worms. Conclusions The gene has a typical characteristics of Wnt frizzled family proteins, the similarity is best to Xenopus laevis. By comparison analysis to speculate as Fz5 gene, we denominate this gene as ＂sjFz5＂ （GenBank accession No. EU370926）. SjFz5 has differential expressions during different developmental stages.