在蔗糖水溶液中加入不同浓度的生姜精油饲喂野生型黑腹果蝇成虫,用时间-剂量-死亡率模型对96 h内各处理果蝇成虫累计死亡率随时间、剂量的变化进行拟合.结果表明:较高浓度的生姜精油对果蝇成虫具有较强的急性毒性.0.5%生姜精油作用72 h, 果蝇成虫的累计死亡率分别为雌88%、雄79%;1%和10%生姜精油作用72 h, 雌雄成虫的累计死亡率均达100%. 随着处理时间的延长,达到50%致死水平所需生姜精油浓度(即LC50)逐渐降低.Tween-20对果蝇成虫(尤其是雄虫)也有较强的毒性,高浓度时作用尤为明显.
Drosophila melanogaster adults were fed with different concentration ginger essential oil, and time-dosage-mortality model was used to simulate their cumulative mortality within 96 h. The results indicated that higher concentrations of ginger essential oil had high acute toxicity to D. melanogaster adults. Seventy-two hours after treated with 0.5% ginger essential oil, the cumulative mortality of the adults was 88% for female and 79% for male, while with 1% and 10% ginger essential oil, the cumulative mortality of both female and male adults was up to 100%. The LC50 of ginger essential oil decreased gradually with increasing time. Tween-20 as an emulsifying agent also had high toxicity to D. melanogaster adults, especially to male adults, which was more obvious in high concentrations.